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Verfahren Der Immunlokalisation: Ein Überblick
Paperback
Series: Essentials
BiologyChemistryGeneral Science
ISBN13: 9783658038281
Publisher: Springer Spektrum
Published: Dec 17 2013
Pages: 47
Weight: 0.17
Height: 0.12 Width: 5.83 Depth: 8.27
Language: German
Der Beitrag von Maria Mulisch bietet einen Überblick über die Techniken der Immunmarkierung für licht- und elektronenmikroskopische Präparate. Es werden Grundlagen der Eigenschaften, Auswahl, Reinigung und Auswahl von Antikörpern vermittelt und verschiedene Detektionssysteme (Enzyme, Fluorochrome, kolloidales Gold) und Signalverstärkungsmethoden vorgestellt und durch Tabellen ergänzt. Probleme und Fehlerquellen sind tabellarisch zusammengefasst und bieten übersichtlich Lösungsmöglichkeiten. Ausgewählte, detaillierte Anleitungen ergänzen und veranschaulichen den Text.
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