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Nukleinsäuren, Konzentration einer DNA-, RNA- und Proteinstocklösung. Ein Praktikumsprotokoll
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ISBN10: 3668142513
ISBN13: 9783668142510
Publisher: Grin Verlag
Published: Feb 17 2016
Pages: 36
Weight: 0.13
Height: 0.09 Width: 5.83 Depth: 8.27
Language: German
ISBN13: 9783668142510
Publisher: Grin Verlag
Published: Feb 17 2016
Pages: 36
Weight: 0.13
Height: 0.09 Width: 5.83 Depth: 8.27
Language: German
Praktikumsbericht / -arbeit aus dem Jahr 2008 im Fachbereich Biologie - Mikrobiologie, Molekularbiologie, Note: 2,3, Ruprecht-Karls-Universität Heidelberg (Fakultät für Biowissenschaften), Veranstaltung: Hauptpraktikum E1: Nukleinsäuren, Sprache: Deutsch, Abstract: Ziel des Versuches ist es, die Konzentration einer DNA-, RNA- und Proteinstocklösung durch Photometrie zu bestimmen. Au erdem soll noch die Reinheit ausgerechnet werden. Inhalt: Konzentrationsbestimmung von DNA-, RNA- und Proteinlösungen Versuchsabsicht Materialien und Methoden Ergebnisse & Fragen Diskussion Plasmididentifizierung: Grundlegende molekularbiologische Methoden Versuchsabsicht Materialien und Methoden Ergebnisse Diskussion & Fragen Klonierung von GFP in E. coli Versuchsabsicht Materialien und Methoden Ergebnisse Diskussion & Fragen Retrovirale Transduktion von CHO-Zellen Versuchsabsicht Materialien und Methoden Ergebnisse Diskussion & Fragen cDNA-Produktion aus mRNA von HeLa-Zellen und Klonierung in E.coli Versuchsabsicht Materialien und Methoden Ergebnisse Diskussion & Fragen RNA-Interference mittels siRNA in HeLa-Zellen Versuchsabsicht Materialien und Methoden Ergebnisse Diskussion & Fragen Anhang
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