Open Daily: 10am - 10pm | Alley door pickup 10am - 7pm 3038 Hennepin Ave Minneapolis, MN
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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Genetic Library Construction and Screening: Advanced Techniques and Applications

Genetic Library Construction and Screening: Advanced Techniques and Applications

Smith, Bruce F.
Bird, R. C.

Paperback

Series: Springer Lab Manuals

Medical ReferenceBiologyChemistry

ISBN10: 364247733X
ISBN13: 9783642477331
Publisher: Springer
Published: Nov 6 2012
Pages: 241
Weight: 0.80
Height: 0.54 Width: 6.14 Depth: 9.21
Language: English
Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice.

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