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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Gene Delivery to Mammalian Cells: Volume 2: Viral Gene Transfer Techniques

Gene Delivery to Mammalian Cells: Volume 2: Viral Gene Transfer Techniques

Heiser, William C.

Paperback

Series: Methods in Molecular Biology, Book 246

Medical ReferenceAnatomy & PhysiologyBiology

ISBN10: 1617373532
ISBN13: 9781617373534
Publisher: Springer Nature
Published: Nov 10 2010
Pages: 566
Weight: 1.70
Height: 1.18 Width: 6.00 Depth: 9.00
Language: English
The efficiency of delivering DNA into mammalian cells has increased t- mendously since DEAE dextran was first shown to be capable of enhancing transfer of RNA into mammalian cells in culture. Not only have other chemical methods been developed and refined, but also very efficient physical and viral delivery methods have been established. The technique of introducing DNA into cells has developed from transfecting tissue culture cells to delivering DNA to specific cell types and organs in vivo. Moreover, two important areas of biology--assessment of gene function and gene therapy--require succe- ful DNA delivery to cells, driving the practical need to increase the efficiency and efficacy of gene transfer both in vitro and in vivo. TM These two volumes of the Methods in Molecular Biology series, Gene Del- ery to Mammalian Cells, are designed as a compendium of those techniques that have proven most useful in the expanding field of gene transfer in mammalian cells. It is intended that these volumes will provide a thorough background on chemical, physical, and viral methods of gene delivery, a synopsis of the myriad techniques currently available to introduce genes into mammalian cells, as well as a practical guide on how to accomplish this. It is my expectation that it will be useful to the novice in the field as well as to the scientist with expertise in gene delivery.

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