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G Protein-Coupled Receptor Screening Assays: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 2268
Medical ReferenceBiologyChemistry
ISBN13: 9781071612200
Publisher: Humana Pr
Published: Jun 5 2021
Pages: 327
Weight: 1.70
Height: 0.80 Width: 7.80 Depth: 10.20
Language: English
1. Isolation of Lipid Rafts by the Detergent and Non-Detergent Based Methods for Localization of GPCRs with Immunoblotting and Laser Scanning Confocal Microscopy
Peter Abdelmaseeh, Andrew C. Tiu, Selim Rozyyev, Laureano D. Asico, Pedro A. Jose, Van Anthony M. Villar
2. Detection of GPCR mRNA Expression in Primary Cells via qPCR, Microarrays, and RNA-Sequencing
Krishna Sriram, Cristina Salmerón, Anna Di Nardo, and Paul A. Insel
3. Construction of Recombinant Cell Lines for GPCR Expression
Philip J. Reeves
4. Recombinant Expression and Purification of Cannabinoid Receptor CB2, a G Protein-Coupled Receptor
Alexei A. Yeliseev
5. Screening for Serotonin Receptor 4 Agonists Using GPCR-Based Sensor in Yeast
Emily A. Yasi and Pamela Peralta-Yahya
6. Immobilization of Olfactory Receptors Carried by Nanosomes onto a Gold Sensor Surface
Jasmina Vidic and Yanxia Hou
7. Screening Methods for Cell-Free Synthesized GPCR/Nanoparticle Samples
Zoe Köck, Volker Dötsch, and Frank Bernhard
8. Fluorescence Anisotropy-Based Assay for Characterization of Ligand Binding Dynamics to GPCRs: The Case of Cy3B-Labelled Ligands Binding to MC4 Receptors in Budded Baculoviruses
Santa Veiksina, Maris-Johanna Tahk, Tõnis Laasfeld, Reet Link, Sergei Kopanchuk, and Ago Rinken
9. Bioluminescence in G Protein-Coupled Receptors Drug Screening Using Nanoluciferase and Halo-Tag Technology
Hannes Schihada, Katarina Nemec, Martin J. Lohse, and Isabella Maiellaro
10. Nanoluciferase-Based Complementation Assay to Detect GPCR-G Protein Interaction
Céline Laschet and Julien Hanson
11. Imaging of Genetically-Encoded FRET-Based Biosensors to Detect GPCR Activity
Luca Bordes, Sergei Chavez-Abiega, and Joachim Goedhart
12. cAMP Biosensor Assay Using BacMam Expression System: Studying the Downstream Signaling of LH/hCG Receptor Activation
Darja Lavogina, Tõnis Laasfeld, Maris-Johanna Tahk, Olga Kukk, Anni Allikalt, Sergei Kopanchuk, and Ago Rinken
13. FLIPR Calcium Mobilization Assays in GPCR Drug Discovery
Grzegorz Woszczek, Elisabeth Fuerst, and Thomas J.A. Maguire
14. Live Cell Imaging and Optogenetics-Based Assays for GPCR Activity
Xenia Meshik and N. Gautam
15. Split-Tobacco Etch Virus (Split-TEV) Method in G Protein-Coupled Receptor Interacting Proteins
Marta Alonso-Gardón and Raúl Estévez
16. NanoLuc-Based Methods to Measure β-Arrestin2 Recruitment to G Protein-Coupled
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