Open Daily: 10am - 10pm | Alley door pickup 10am - 7pm 3038 Hennepin Ave Minneapolis, MN
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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Fed-Batch Fermentation: A Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli

Fed-Batch Fermentation: A Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli

Moulton, G. G.

Hardcover

Series: Woodhead Publishing Biomedicine

Medical ReferenceBiologyChemistry

ISBN10: 1907568921
ISBN13: 9781907568923
Publisher: Academic Pr Inc
Published: Oct 29 2014
Pages: 188
Weight: 0.97
Height: 0.50 Width: 6.14 Depth: 9.21
Language: English
Fed-batch Fermentation is primarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision. This process can also be done on a small (2L) scale that is scalable to 30L or more. All reagents (media, carbon source, plasmid vector and host cell) used are widely available and are relatively inexpensive. This method has been used to produce three different protein products following cGMP guidelines for Phase I clinical studies.

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