Open Daily: 10am - 10pm | Alley door pickup 10am - 7pm 3038 Hennepin Ave Minneapolis, MN
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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Fundamentals of Molecular Techniques: From DNA to Genomics

Fundamentals of Molecular Techniques: From DNA to Genomics

Kurian, Anoja

Paperback

Biology

ISBN10: 6206772586
ISBN13: 9786206772583
Publisher: Scholars' Press
Published: Jun 19 2024
Pages: 72
Weight: 0.26
Height: 0.17 Width: 6.00 Depth: 9.00
Language: English
This book delves into essential molecular biology techniques that are pivotal for contemporary research and medical diagnostics. It covers the basics of extracting genetic material, which facilitates genetic analysis, gene expression studies, and forensic applications. Detailed explanations are provided for the principles and workings of the Polymerase Chain Reaction (PCR) and its variants, including quantitative PCR, reverse transcription PCR, as well as nested, multiplex, hotstart PCR, and other major types. Blotting techniques such as Southern, Northern, and Western blots are thoroughly discussed for their role in detecting specific nucleic acids or proteins. The book also simplifies the CRISPR gene editing technology. Sequencing techniques are comprehensively reviewed, with a comparison between traditional Sanger sequencing and Next-Generation Sequencing (NGS) technologies like Ion Torrent, Pyrosequencing, Nanopore, and Illumina sequencing. Their applications in genome analysis, disease diagnosis, microbial diversity, and evolutionary studies are highlighted. It is an indispensable resource for scientists, researchers, and students in the field of molecular biology.

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