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Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing

Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing

Paperback

Medical ReferenceBiologyGeneral Science

ISBN10: 3319810189
ISBN13: 9783319810188
Publisher: Springer
Published: May 30 2018
Pages: 399
Weight: 1.27
Height: 0.84 Width: 6.14 Depth: 9.21
Language: English
The High Throughput Sequencing technologies triple-W discussion: Why to use HTS, What is the optimal HTS method to use, Which data analysis workflow to follow.- Whole genome sequencing recommendations.- Counseling for targeted DNA region re-sequencing.- Transcriptome profiling strategies.- Differential mRNA Alternative Splicing.- microRNA discovery and expression analysis in animals.- Analysis of Long Non-coding RNAs in RNA-seq data.- Ribosome profiling.- Genome-wide analysis of DNA methylation patterns by high-throughput sequencing.- Characterization of DNA-protein interactions: Design and analysis of ChIP-seq experiments.- PAR-CLIP: A genomic technique to dissect RNA-protein interactions.- Metagenomic design and sequencing.- A hitchhiker's guide to Metatranscriptomics.- Eukaryotic Single-Cell mRNA Sequencing.- Eukaryotic Single-Cell DNA Sequencing.- Submitting data to a public repository, the final step of a successful HTS experiment.

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