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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Fibrosis Research: Methods and Protocols

Fibrosis Research: Methods and Protocols

Varga, John
Brenner, David A.
Phan, Sem H.

Hardcover

Series: Methods in Molecular Medicine, Book 117

Medical Reference

ISBN10: 158829479X
ISBN13: 9781588294791
Publisher: Springer Nature
Published: Aug 8 2005
Pages: 392
Weight: 1.69
Height: 1.05 Width: 6.28 Depth: 9.38
Language: English
Leading investigators review the highlights of current fibrosis research and the experimental methodologies used uncover the mechanisms that drive it. In their discussion of research methodologies utilizing cultured cells to model various aspects of the fibrotic response in vitro, the authors describe the isolation, characterization, and propagation of mesenchymal cells, and highlight the similarities and differences between methods that are appropriate for different types of fibroblasts. Approaches for studying collagen gene regulation and TGF-b production are also discussed, along with experimental methodologies utilizing animal models to study the pathogenesis of fibrosis. The protocols follow the successful Methods in Molecular Medicine(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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