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Estrogen Receptors: Methods and Protocols

Estrogen Receptors: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 2418

Anatomy & Physiology

ISBN10: 1071619195
ISBN13: 9781071619193
Publisher: Humana Pr
Published: Feb 5 2022
Pages: 427
Weight: 1.90
Height: 1.10 Width: 7.40 Depth: 10.33
Language: English
This fully updated collection includes chapters on a wide array of techniques that are vital for advancing our understanding of the physiological and pathological effects of estrogen, mediated by estrogen receptor α (ERα) and estrogen receptor β (ERβ) as well as the non-genomic membrane-bound G-protein coupled estrogen receptor (GPER/GPR30). The protocols range from standard methods and important laboratory workhorses such as immunoblot and RT-qPCR, to newer technologies such as PET imaging, reporter animal models, polysome fractionation, and many others, involving a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written for this highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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