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Epigenome Editing: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1767
Medical ReferenceOther Animals
ISBN13: 9781493977734
Publisher: Humana Pr
Published: Mar 10 2018
Pages: 482
Weight: 2.37
Height: 1.06 Width: 7.00 Depth: 10.00
Language: English
Part I: General Reviews
1. Editing the Epigenome: Overview, Open Questions, and Directions of Future Development
Marianne G. Rots and Albert Jeltsch
2. Zinc Fingers, TALEs and CRISPR Systems: A Comparison of Tools for Epigenome Editing
Charlene Babra Waryah, Colette Moses, Mahira Arooj, and Pilar Blancafort
3. Designing Epigenome Editors: Considerations of Biochemical and Locus Specificities
Dilara Sen and Albert J. Keung
4. Generation of TALE-Based Designer Epigenome Modifiers
Sandra Nitsch and Claudio Mussolino
Part II: Topical Reviews
5. Neuroepigenetic Editing
Peter J. Hamilton, Carissa J. Lim, Eric J. Nestler, and Elizabeth A. Heller
6. Allele-Specific Epigenome Editing
Pavel Bashtrykov and Albert Jeltsch
7. Key to Delivery: The (Epi-)Genome Editing Vector Toolbox
Sabrina Just and Hildegard Büning
8. CRISPR/dCas9 Switch Systems for Temporal Transcriptional Control
Rutger A.F. Gjaltema and Edda G. Schulz
Part III: Delivery Protocols
9. Delivery of Designer Epigenome Modifiers into Primary Human T Cells
Tafadzwa Mlambo, Marianna Romito, Tatjana I. Cornu, and Claudio Mussolino
10. Viral Expression of Epigenome Editing Tools in Rodent Brain Using Stereotaxic Surgery Techniques
Peter J. Hamilton, Carissa J. Lim, Eric J. Nestler, and Elizabeth A. Heller
11. Stable Expression of Epigenome Editors Via Viral Delivery and Genomic Integration
Carolin Kroll and Philipp Rathert
12. Purified-Protein Delivery to Activate an Epigenetically-Silenced Allele in Mouse Brain
Benjamin Pyles, Barbara J. Bailus, Henriette O'Geen, and David J. Segal
13. Non-Viral Methodology for Efficient Co-Transfection
Jessica A. Kretzmann, Cameron W. Evans, Marck Norret, Pilar Blancafort, and K. Swaminathan Iyer
Part IV: Histone Editing Readout Protocols
14. Chromatin Immunoprecipitation in Human and Yeast Cells
Jessica B. Lee and Albert J. Keung
15. Chromatin Immunoprecipitation and High Throughput Sequencing (ChIP-Seq): Tips and Tricks Regarding the Laboratory Protocol and Initial Downstream Data Analysis
Darren K. Patten, Giacomo Corleone, and Luca Magnani
Part V: DNA Methylation Editing Readout Protocols
16. Generation of Whole Genome Bisulfite Sequencing Libraries for Comprehensive DNA Methylome Analysis
Dulce B. Vargas-Landin, Jahnvi Pflüger, and Ryan Lister
17. Approaches for the Analysis and Interpretation of Whole Genome Bisulfite Sequencing Data
Tim Stuart, Sam Buckberry, and Ryan Lister
18. Whole Genome Bisulfite Sequencing for the Analysis of Genome-Wide DNA Methylation and Hydroxymethylation Patterns at Single-Nucleotide Resolution
Magali Kernaleguen, Christian Daviaud, Yimin Shen, Eric Bonnet, Victor Renault, Jean-François Deleuze, Florence Mauger, and Jörg Tost
19. Locus-Specific DNA Methylation Analysis by Targeted Deep Bisulfite Sequencing
Elsa Leitão, Jasmin Beygo, Michael Zeschnigk, Ludger Klein-Hitpass, Marcel Bargull, Sven Rahmann, and Bernhard Horsthemke
20. DNA Methylation Analysis by Bisulfite Conversion Coupled to Double Multipl
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