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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Electroporation Protocols for Microorganisms

Electroporation Protocols for Microorganisms

Nickoloff, Jac A.

Paperback

Series: Methods in Molecular Biology, Book 47

Medical ReferenceBiologyEvolution

ISBN10: 0896033104
ISBN13: 9780896033108
Publisher: Springer Nature
Published: Jun 20 1995
Pages: 372
Weight: 1.28
Height: 0.88 Width: 6.00 Depth: 9.00
Language: English
Electroporation is one of the most widespread techniques used in modem molecular genetics. It is most commonly used to introduce DNA into cells for investigations of gene structure and function, and in this regard, electroporation is both highly versatile, being effective with nearly all species and cell types, and highly efficient. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. However, exposure of cells to brief, hi- intensity electric fields has found broad application in other aspects of biological research, and is now routinely used to introduce other types of biological and analytic molecules into cells, to induce cell-cell fusion, and to transfer DNA directly between different species. The first seven chapters of Electroporation Protocols for Micro- organisms describe the underlying theory of electroporation, the com- mercially available instrumentation, and a number of specialized electroporation applications, such as cDNA library construction and interspecies DNA electrotransfer. Each of the remaining chapters pre- sents a well developed method for electrotransformation of a particular bacterial, fungal, or protist species. These chapters also serve to intro- duce those new to the field the important research questions that are currently being addressed with particular organisms, highlighting both the major advantages and limitations of each species as a model organ- ism, and explaining the roles that electroporation has played in the development of the molecular genetic systems currently in use.

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