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Experimental Models of Parkinson's Disease
Hardcover
Series: Methods in Molecular Biology, Book 2322
Medical ReferenceBiologyCognition
ISBN13: 9781071614945
Publisher: Humana
Published: May 28 2021
Pages: 217
Weight: 1.30
Height: 0.70 Width: 7.80 Depth: 10.20
Language: English
Part I: Biochemical Experiments and Cellular Models of Parkinson's Disease
1. α-Synuclein Seeding Assay Using RT-QuIC
Ayami Okuzumi, Taku Hatano, Takeshi Fukuhara, Shinichi Ueno, Nobuyuki Nukina, Yuzuru Imai, and Nobutaka Hattori
2. Electron-Microscopic Analysis of α-Synuclein Fibrils
Airi Tarutani and Masato Hasegawa
3. α-Synuclein Seeding Assay Using Cultured Cells
Jun Ogata, Daisaku Takemoto, Shotaro Shimonaka, Yuzuru Imai, and Nobutaka Hattori
4. Analysis of α-Synuclein in Exosomes
Taiji Tsunemi, Yuta Ishiguro, Asako Yoroisaka, and Nobutaka Hattori
5. Measurement of GCase Activity in Cultured Cells
Yuri Shojima, Jun Ogata, Taiji Tsunemi, Yuzuru Imai, and Nobutaka Hattori
6. Detection of Substrate Phosphorylation of LRRK2 in Tissues and Cultured Cells
Kyohei Ito, Lejia Xu, Genta Ito, and Taisuke Tomita
7. Two Methods to Analyze LRRK2 Functions Under Lysosomal Stress: The Measurements of Cathepsin Release and Lysosomal Enlargement
Maria Sakurai and Tomoki Kuwahara
8. Differentiation of Midbrain Dopaminergic Neurons from Human iPS Cells
Kei-ichi Ishikawa, Risa Nonaka, and Wado Akamatsu
9. Monitoring PINK1-Parkin Signaling Using Dopaminergic Neurons from iPS Cells
Kahori Shiba-Fukushima and Yuzuru Imai
Part II: Mammalian Models of Parkinson's Disease
10. Generation of Mitochondrial Toxin Rodent Models of Parkinson's Disease Using 6-OHDA, MPTP, and Rotenone
Hiroharu Maegawa and Hitoshi Niwa
11. Midbrain Slice Culture as an Ex Vivo Analysis Platform for Parkinson's Disease
Yuji Kamikubo, Keiko Wakisaka, Yuzuru Imai, and Takashi Sakurai
12. α-Synuclein Propagation Mouse Models of Parkinson's Disease
Norihito Uemura, Jun Ueda, Shinya Okuda, Masanori Sawamura, and Ryosuke Takahashi
13. Common Marmoset Model of α-Synuclein Propagation
Masami Masuda-Suzukake, Aki Shimozawa, Masashi Hashimoto, and Masato Hasegawa
14. Application of a Tissue Clearing Method for the Analysis of Dopaminergic Axonal Projections
Kenta Yamauchi, Megumu Takahashi, and Hiroyuki Hioki
15. Deep Brain Stimulation Using Animal Models of Parkinson's Disease
Asuka Nakajima and Yasushi Shimo
Part III: Invertebrate Models of Parkinson's Disease
16. Assessment of Cytotoxicity of α-Synuclein in Budding Yeast Using a Spot Growth Assay and Fluorescent Microscopy
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