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Exon Skipping: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 867
ISBN13: 9781617797668
Publisher: Humana
Published: Mar 28 2012
Pages: 440
Weight: 2.16
Height: 1.15 Width: 7.32 Depth: 10.29
Language: English
Part I: Mutations
1. DNA Diagnostics and Exon Skipping
Umasuthan Srirangalingam and Shern L. Chew
2. Bioinformatics and Mutations Leading to Exon Skipping
F.O. Desmet and C. Béroud
3. Minigenes to Confirm Exon Skipping Mutations
Lourdes R. Desviat, Belén Pérez & Magdalena Ugarte
4.Analysis and Interpretation of RNA Splicing Alterations in Genes Involved in Genetic Disorders
Maaike P.G. Vreeswijk and Heleen M. van der Klift
5. Exon Skipping Mutations in Neurofibromatosis
Emanuele Buratti and Diana Baralle
Part II: Tools to Induce Exon Skipping
6. Overview on Applications of Antisense-mediated Exon Skipping
Willeke van Roon-Mom and Annemieke Aartsma-Rus
7. Overview on DMD Exon Skipping
Annemieke Aartsma-Rus
8. Overview on AON Design
Annemieke Aartsma-Rus
9. Optimizing RNA/ENA Chimeric Antisense Oligonucleotides Using in vitro Splicing
Yasuhiro Takeshima, Mariko Yagi, Masafumi Matsuo
10. Optimizing Antisense Oligonucleotides using Phosphorodiamidate Morpholino Oligomers
Linda J Popplewell, Alberto Malerba, and George Dickson
11. Optimizing Splice Switching Oligomer Sequences using 2'-O-methyl Phosphorothioate Chemistry
Carl Adkin, Sue Fletcher and Steve D Wilton
12. Exon Skipping Quantification by Real Time PCR
Alessandra Ferlini and Paola Rimessi
13. Antisense-mediated Exon Skipping to Shift Alternative Splicing to Treat Cancer
Jing Wan
14. Antisense-mediated Exon Skipping to Generate Soluble Receptors
A. Seda Yilmaz-Elis and J. Sjef Verbeek
15. Antisense-mediated Exon Skipping to Reframe Transcripts
Sandrina Turczynski, Matthias Titeux, Nathalie Pironon, and Alain Hovnanian
16. U1 snRNA as an Effective Vector for Stable Expression of Antisense Molecules andfor the Inhibition of the Splicing Reaction
Martone Julie, De Angelis Fernanda Gabriella and Bozzoni Irene
17. Engineering U7snRNA Gene to Reframe Transcripts
Aurélie Goyenvalle
18. Dynamic Fluorescent and Luminescent Reporters for Cell Based Splicing Screens
Claude C. Warzecha, Ruben Hovhannisyan, and Russ P. Carstens
19. Antisense-mediated Exon Skipping to Induce Gene Knockdown
Petra Disterer and Bernard Khoo
20. Antisense-mediated Exon Inclusion
Yimin Hua and Adrian R. Krainer
21. Antisense Genes to Induce Exon Inclusion
R. Nlend Nlend & D. Schümperli22. Using Mini-genes to Identify Factors that Modulate Alternative Splicing
Robert Morse, Adrian G. Todd and Philip J. Young
Part III: Delivery
23. Overview of Alternative Oligonucleotide Chemistries for Exon Skipping
Amer F. Saleh, Andrey A. Arzumanov and Michael J. Gait
24. Identification of Peptides for Tissue Specific Delivery
Hans Heemskerk
25. Systemic Delivery of Antisense Oligomer in Animal Models and its Implications for Treating DMD
Qi Long Lu and Bo Wu
26. Cell-penetrating Peptides Enhance Systemic Delivery of Antisense Morpholino Oligomers
Hong M Moulton
27. Optimizing Tissue-specific Antisense Oligonucleotide-peptide Conjugates
Corinne A Betts, Suzan M Hammond, Hai-fang Yin, and Matthew JA Wood
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