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The Escrt Complexes: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 1998
ISBN13: 9781493994946
Publisher: Humana
Published: Aug 14 2020
Pages: 322
Weight: 1.30
Height: 0.71 Width: 7.00 Depth: 10.00
Language: English
1. Analysis of the Archaeal ESCRT Apparatus
Rachel Y. Samson, Iain G. Duggin, and Stephen D. Bell
2. Genetic and Cell Biology Methods to Study ESCRTs in Drosophila melanogaster
Marco Gualtieri and Thomas Vaccari
3. Functional Analysis of ESCRT-Positive Extracellular Vesicles in the Drosophila Wing Imaginal Disc
Tamas Matusek, Pascal Thérond, and Maximilian Fürthauer
4. Subcellular Localization of ESCRT-II in the Nematode C. elegans by Correlative-Light Electron Microscopy
Céline Largeau, Emmanuel Culetto, and Renaud Legouis
5. Proximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected Cells
Binod Kumar, Mohanan Valiya Veettil, Arunava Roy, and Bala Chandran
6. Immuno-Localization of ESCRT Proteins in Virus-Infected Cells by Fluorescence and Electron Microscopy
Keisuke Tabata, Atsuki Nara, Hiroko Omori, and Eiji Morita
7. Single Cell Fluorescence Ratio Image Analysis for Studying ESCRT Function in Receptor Trafficking
Jalal M. Kazan, Gergely L. Lukacs, Pirjo M. Apaja, and Arnim Pause
8. Genetic and Biochemical Analyses of Yeast ESCRT
Sudeep Banjade, Shaogeng Tang, and Scott D. Emr
9. Live Imaging of ESCRT Proteins in Microfluidically-Isolated Hippocampal Axons
Veronica Birdsall, Jose C. Martinez, Lisa Randolph, Ulrich Hengst, and Clarissa L. Waites
10. Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High Resolution Imaging Techniques SIM and Cryo-SXT
Shai Adar-Levor, Inna Goliand, Michael Elbaum, and Natalie Elia
11. Three Dimensional Surface Rendering of ESCRT Proteins Microscopy Data Using UCSF Chimera Software
Romain Le Bars, Michele W. Bianchi, and Christophe Lefebvre
12. Transient Expression of ESCRT-Components in Arabidopsis Root Cell Suspension Culture-Derived Protoplasts
Marie-Kristin Nagel, Karin Vogel, and Erika Isono
13. Crystallization and Biophysical Approaches for Studying the Interactions between the Vps4-MIT Domain and ESCRT-III Proteins
Takayuki Obita, Rieko Kojima, and Mineyuki Mizuguchi
14. Biochemical Approaches to Studying C. elegans ESCRT Function In Vitro
Samuel Block, Amber L. Schuh, and Anjon Audhya
15. Purification of Recombinant ESCRT-III Proteins and Their Use in Atomic Force Microscopy and In Vitro Binding and Phosphorylation Assays
Luisa Capalbo, Ioanna Mela, Maria Alba Abad, A. Arockia Jeyaprakash, J. Michael Edwardson, and Pier Paolo D'Avino
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