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Epigenetic Reprogramming During Mouse Embryogenesis: Methods and Protocols

Epigenetic Reprogramming During Mouse Embryogenesis: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 2214

BiologyGenetics

ISBN10: 1071609572
ISBN13: 9781071609576
Publisher: Humana Pr
Published: Sep 19 2020
Pages: 318
Weight: 1.60
Height: 0.85 Width: 7.24 Depth: 10.13
Language: English

This volume explores the latest techniques used to study and understand chromatin reprogramming in embryos and germ cells. Various culture systems are presented, which consist of invaluable tools to investigate many developmental processes. This book also looks at methods for direct examination of DNA, RNA, and proteins in embryos, along with low-input and single-cell assays for exploring these features at the genome-wide scale. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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