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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Development of a Fully Integrated "Sample-In-Answer-Out" System for Automatic Genetic Analysis

Development of a Fully Integrated "Sample-In-Answer-Out" System for Automatic Genetic Analysis

Zhuang, Bin

Paperback

Series: Springer Theses

Technology & EngineeringChemistryGenetics

ISBN10: 9811352208
ISBN13: 9789811352201
Publisher: Springer Nature
Published: Feb 9 2019
Pages: 114
Weight: 0.45
Height: 0.30 Width: 6.14 Depth: 9.21
Language: English

This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an in-situ PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system's performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successfuldevelopment and operation of this microsystem establishes the feasibility of rapid sample-in-answer-out testing in routine clinical practice.

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