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Decellularization Methods of Tissue and Whole Organ in Tissue Engineering
Hardcover
Series: Advances in Experimental Medicine and Biology, Book 1345
ISBN13: 9783030827342
Publisher: Springer Nature
Published: Sep 29 2021
Pages: 259
Weight: 1.54
Height: 0.69 Width: 7.00 Depth: 10.00
Language: English
Preface
Acknowledgment
Section 1: Introduction of Acellularization
Section 2: Extra Cellular Matrix (ECM) as a Bioscaffold
Section 3: Methods of Characterization of Acellularized Tissue
Histology Staining: H&E, Masson's Trichrome Staining, DAPI Staining
Determination amount of Hydroxy lysin and Hydroxy Proline
DNA Quantification
Scanning Electron Microscopy (SEM)
Mechanical Strength (Tensile and Compressive test)
Sodium Dodecyl Sulfate (SDS) Detection Method
Cytotoxicity Assessment: Live/Dead Assay, MTT Assay
Cell Attachment and Cell Proliferation
CT Angiography
Section 4: Methods of Sterilization of Acellularized Tissue
Section 5: skin
Chapter 1: Thin Skin
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Chapter 2: Thick Skin
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Section 6: Cartilage Tissue
Chapter 1: Hyaline cartilage
History
Materials & MethodsCell seeding
Clinical Applications
Limitations
Conclusion
Chapter 2: Fibrocartilage
History
Materials & MethodsCell seeding
Clinical Applications
Limitations
Conclusion
Chapter 3: Elastic cartilage
History
Materials & MethodsCell seeding
Clinical Applications
Limitations
Conclusion
Section 7: Bone Tissue
History
Materials & MethodsCell seeding
Clinical Applications
Limitations
Conclusion
Section 8: Cardiovascular System
History
Materials & MethodsCell seeding
Clinical Applications
Limitations
Conclusion
Section 9: Digestive System I
Chapter 1: Tongue
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Chapter 2: Esophagus
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
Chapter 3: Stomach
History
Materials & Methods
Cell seeding
Clinical Applications
Limitations
Conclusion
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