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DNA Modifications: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 2198
BiologyGeneral ScienceGenetics
ISBN13: 9781071608784
Publisher: Humana
Published: Aug 22 2021
Pages: 495
Weight: 1.94
Height: 1.03 Width: 7.00 Depth: 10.00
Language: English
Part I: Introduction
1. Modified Forms of Cytosine in Eukaryotes: DNA (De)Methylation and Beyond
Maria Eleftheriou and Alexey Ruzov
2. Evidence for Non-Cytosine Epigenetic DNA Modifications in Multicellular Eukaryotes: An Overview
Paige Lowe, Ryszard Olinski, and Alexey Ruzov
3. Mass Spectrometry-Based Analysis of DNA Modifications: Potential Applications in Basic Research and Clinic
Ryszard Olinski, Rafal Rozalski, and Daniel Gackowski
4. Mapping DNA Methylation in Mammals: The State of the Art
Antonio Lentini and Colm E. Nestor
Part II: Detection and Quantification of DNA Modifications Using Mass Spectrometry and Capillary Gel Electrophoresis
5. Detection of Modified Bases in Bacteriophage Genomic DNA
Yan-Jiun Lee and Peter R. Weigele
6. Liquid Chromatography-Mass Spectrometry Analysis of Cytosine Modifications
Nan Dai and Ivan R. Corrêa Jr.
7. Detection of DNA Methylation in Genomic DNA by UHPLC-MS/MS
Konstantinos Boulias and Eric Lieberman Greer
8. Quantification of DNA Modifications Using Two-Dimensional Ultra-Performance Liquid Chromatography Tandem Mass-Spectrometry (2D-UPLC-MS/MS)
Marta Starczak, Maciej Gawronski, Ryszard Olinski, and Daniel Gackowski
9. MS Analysis of DNA Modifications in Urinary/Body Fluids
Aleksandra Skalska, Agnieszka Siomek-Gorecka, Ryszard Olinski, and Rafal Rozalski
10. Preparation of Internal Standards for 2D-UPLC-MS/MS Quantification of Non-Canonical DNA Bases
Marta Starczak, Aleksandra Skalska, Rafal Rozalski, Ryszard Olinski, and Daniel Gackowski
11. Detection of TET-Oxidized 5-Methylcytosine Bases by Capillary Gel Electrophoresis
Romualdas Vaisvila, Lise Hunault, and Lana Saleh
Part III: Assessing the Levels and Spatial Distribution of DNA Modifications Using Antibody-Based Approaches
12. Immunochemical Detection of Modified Cytosine Species in Mammalian Preimplantation Embryos
Tie-Bo Zeng and Piroska E. Szabó
13. Immunochemical Detection of Modified Cytosine Species in Lampbrush Chromatin
Garry T. Morgan
14. Detection of Low-Abundance DNA Modifications Using Signal Amplification-Based Immunocytochemistry
Abdulkadir Abakir and Alexey Ruzov
15. Immunohistochemical Detection of Modified Cytosine Bases in Rodent Brain
Marcus D. Wallis and Rebecca C. Trueman
16. Immunohistochemical Detection of 5-Hydroxymethylcytosine and 5-Ca
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