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Dengue Virus: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 2409
ISBN13: 9781071618813
Publisher: Humana
Published: Oct 29 2022
Pages: 295
Weight: 1.18
Height: 0.65 Width: 7.00 Depth: 10.00
Language: English
Part I: Virus and Virus Protein Production
1. Virus Propagation and Plaque Assay for Dengue Virus
Pedro Henrique Carneiro, Lucas Mendes-Monteiro, and Ronaldo Mohana-Borges
2. Transmission Electron Microscopy Studies of Dengue Viruses
3. Production of Properly Folded NS1 Protein in Bacterial Cells
Diego Allonso
4. Expression and Purification of Dengue NS1 Protein in Sf9-Baculovirus System
Diego R. Coelho and Pedro Henrique Carneiro
5. Purification of Dengue and Zika Virus Non-Structural Protein 5 for Crystallization and Screening of Antivirals
Aicha Gharbi-Ayachi, Abbas El Sahili, and Julien Lescar
6. Obtention of Dengue Virus Membrane Proteins and Role for Virus Assembly
Szu-Chia Hsieh, Wen-Yang Tsai, and Wei-Kung Wang
7. Construction of Genomic and Sub-Genomic Dengue Infectious Replicons
Thu M. Cao and Richard J. Kuhn
Part II: Interaction between Dengue Virus and Cellular Proteins
8. Dengue Virus Capsid-Protein Dynamics in Live Infected Cells Studied by Pair Correlation Analysis
Manuela V. Gabriel, Ignacio Sallaberry, Guadalupe S. Costa Navarro, Enrico Gratton, Andrea V. Gamarnik, and Laura C. Estrada
9. Yeast Two-Hybrid System for Mapping Novel Dengue Protein Interactions
Jonas Nascimento Conde
10. Isolation and Identification of Dengue Virus Interactome with Human Plasma Proteins by Affinity Purification-Mass Spectrometry
Vivian Huerta and Yassel Ramos
Part III: Immunopathogensis and Diagnosis
11. Molecular Diagnosis Of Dengue
Priscila C.G. Nunes, Monique R.Q. Lima, and Flávia B. dos Santos
12. Serological Diagnosis of Dengue
Monique R.Q. Lima, Priscila C.G. Nunes, and Flávia B. dos Santos
13. Measuring Transendothelial Electrical Resistance (TEER) for Dengue Infection Studies
Jonas Nascimento Conde, Megan Mladinich, William Schutt, and Erich R. Mackow
14. Evaluation of DENV-Induced Endothelial Cell Permeability by Measurements of Transendothelial Electrical Resistance (TEER) and Extravasation of Proteins and Virus
Lana Monteiro Meuren, Sharton Vinicius Antunes Coelho, and Luciana Barros de Arruda
15. In Vitro Cytokine Production by Dengue-Infected Human Monocyte-Derived Dendritic Cells
Allan Henrique Depieri Cataneo, Juliano Bordignon, Pryscilla Fanini Wowk, and Guilherme Ferreira Silveira
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