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    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Cryotechniques in Biological Electron Microscopy

Cryotechniques in Biological Electron Microscopy

Steinbrecht, Rudolf A.
Zierold, Karl

Paperback

Medical ReferenceBiology

ISBN10: 3642728170
ISBN13: 9783642728174
Publisher: Springer Nature
Published: Dec 6 2011
Pages: 297
Weight: 1.11
Height: 0.66 Width: 6.69 Depth: 9.61
Language: English
To preserve tissue by freezing is an ancient concept going back pre- sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan- ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro- molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos- sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro- duced a wealth of successful applications.

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