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Cryopreservation and Freeze-Drying Protocols

Cryopreservation and Freeze-Drying Protocols

Paperback

Series: Methods in Molecular Biology, Book 1257

Technology & EngineeringBiology

ISBN10: 1493949128
ISBN13: 9781493949120
Publisher: Springer Nature
Published: Sep 22 2016
Pages: 509
Weight: 1.98
Height: 1.05 Width: 7.00 Depth: 10.00
Language: English

This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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