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Colorectal Cancer: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1765
ISBN13: 9781493977642
Publisher: Humana Pr
Published: Mar 28 2018
Pages: 353
Weight: 1.88
Height: 0.81 Width: 7.00 Depth: 10.00
Language: English
Dedication...
Preface...
Table of Contents...
Contributing Authors...
Part I Molecular Basis of Colorectal Cancer
1 Cell Line Models of Molecular Subtypes of Colorectal Cancer
Jennifer Mooi, Ian Luk, and John Mariadason
2 Dissecting Oncogenic RTK Pathways in Colorectal Cancer Initiation and Progression
Stephen McManus, Walid Chababi, Dominique Arsenault, Claire M. Dubois, and Caroline Saucier
Salah Boudjadi, Julie C. Carrier, and Jean-François Beaulieu
4 Identification and Functional Analysis of Gene Regulatory Sequences Interacting with Colorectal Tumor Suppressors
Katja Dahlgaard and Jesper Thorvald Troelsen
5 Methods for In Vivo Functional Studies of Chromatin-Modifying Enzymes in Early Steps of Colon Carcinogenesis
Martine Chevillard-Briet and Fabrice Escaffit
6 The Colorectal Cancer Microenvironment: Strategies for Studying the Role of Cancer Associated Fibroblasts
Rahul Bhome, Massimiliano Mellone, Katherine Emo, Gareth Thomas, Emre Sayan, and Alex H. Mirnezami
7 Methods for Assessing Apoptosis and Anoikis in Normal Intestine/Colon and Colorectal Cancer
Pierre H. Vachon
8 Molecular Analysis of the Microbiome in Colorectal Cancer
Fiona Clegg, Susan H. Berry, Richard Hansen and Georgina L. Hold
9 Proteomic Analysis of Colorectal Cancer Cells
Anaïs Chauvin and François-Michel Boisvert
10 Autophagic Flux Assessment in Colorectal Cancer Cells
Annie Lauzier and Steve Jean
Part II New Developments in Colorectal Cancer Diagnostics and Screening
11 Classification of Colorectal Cancer in Molecular Subtypes by Immunohistochemistry
Sanne ten Hoorn, Anne Trinh, Joan de Jong, Lianne Koens, and Louis Vermeulen
12 Stool DNA Integrity Method for Colorectal Cancer Detection
Claudia Rengucci, Giulia De Maio, Maura Menghi, and Daniele Calistri
13 RT-qPCR for Fecal Mature microRNA Quantification and Validation
Farid E. Ahmed, Nancy C. Ahmed, Mostafa M. Gouda, Paul W. Vos, and Chris Bonnerup
14 A Stool Multi-Target mRNA Assay for the Detection of Colorectal Neoplasms
Elizabeth Herring, Shigeru Kanaoka, Eric Tremblay, and Jean-François Beaulieu
15 Colorectal Cancer Detection Using Targeted LC-MS Metabolic Profiling
Danijel Djukovic, Jiangjiang Zhang, and Daniel Raftery
16 Proteomic Profiling for Colorectal Cancer Biomarker Discovery
Paula Álvarez-Chaver, Loretta De Chiara, and Vicenta Soledad Martínez-Zorzano
17 Tumor-Derived Microparticles to Monitor Colorectal Cancer Evolution
Diane Mege, Laurence Panicot-Dubois, and Christophe Dubois
Part III Recent Advances in Colorectal Cancer Therapeutics
Patrick S. Lin and Thomas J. Semrad
19 Testing Cell-Based Immunotherapy for Colorectal Cancer
Jong Soon Kang, Yeo Dae Yoon, Myeong Youl Lee, Chang Woo Lee, Suk Jong Lee, and Sang-Bae Han
20 Patient-Derived Xenograft Models of Colorectal Cancer: Procedures for Engraftment and Propagation
Danielle M. Burgenske, David J. Monsma, and Jeffrey P. MacKeigan
21 Use of Organoids to Characterize Signaling Pathways in Cancer Initiation
Christina Oatway, Calley L. Hirsch, and Alex Gregorieff
22 Identification of Novel Molecules Targeting Cancer Stem Cells
Yannick D. Benoit
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