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Circulating Tumor Cells: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1634
ISBN13: 9781493971435
Publisher: Humana Pr
Published: Aug 18 2017
Pages: 306
Weight: 1.75
Height: 0.75 Width: 7.00 Depth: 10.00
Language: English
This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Each chapter provides comprehensive descriptions and guidelines on how to perform innovative experiments in CTC research. Included are protocols for capture of CTCs via filtration and density gradient centrifugation; microfluidic and immunomagnetic separation of CTCs; detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, and flow cytometry; assays designed for genomic characterization and functional analyses of CTCs, and many more. Written in the highly successful Methods in Molecular Biology series format, the chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Circula
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