Sign-In
Cart
Cell Line Development
Hardcover
Series: Cell Engineering, Book 6
ISBN13: 9789048122448
Publisher: Springer
Published: Sep 10 2009
Pages: 253
Weight: 1.20
Height: 0.63 Width: 6.14 Depth: 9.21
Language: English
Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This unique advantage outweighs the costs associated with mammalian cell culture, which are far grater in terms of development time and manufacturing when compared to microbial culture. The development of cell lines has undergone several advances over the years, essentially to meet the requirement to cut the time and costs associated with using such a complex hosts as production platforms.
This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout.
1 different editions
Also available
Also from
Al-Rubeai, Mohamed
Also in
Biology
The Age of Living Machines: How Biology Will Build the Next Technology Revolution
Hockfield, Susan
Paperback
Entangled Life: How Fungi Make Our Worlds, Change Our Minds & Shape Our Futures
Sheldrake, Merlin
Paperback
Hidden Creatures: Luscious Leeches, Bashful Botflies, and the Wondrous, History-Shaping World of Parasites
Martins, Dino
Hardcover
Beyond Inheritance: Our Ever-Mutating Cells and a New Understanding of Health
Khamsi, Roxanne
Hardcover
The Biology of Trauma: How the Body Holds Fear, Pain, and Overwhelm, and How to Heal It
Apigian, Aimie
Hardcover
Go Forth and Forage: A Guide to Foraging Over 50 of the Most Common Edible & Medicinal North American Mushrooms
Johnson, Whitney
Paperback
Forbidden Facts: Government Deceit & Suppression about Brain Damage from Childhood Vaccines
de Becker, Gavin
Hardcover
The World Beneath Our Feet: The Hidden Life of Soil and Why It Matters to Us All
Ashwood, Frank
Hardcover
The Power of Life: The Invention of Biology and the Revolutionary Science of Jean-Baptiste Lamarck
Riskin, Jessica
Hardcover
Ernst Haeckel's Art Forms in Nature: A Visual Masterpiece of the Natural World
Haeckel, Ernst
Hardcover
History by a Hair: Thomas Jefferson, Sally Hemings & Solving a 200-Year-Old Mystery
Kurin, Richard
Hardcover
Food Intelligence: The Science of How Food Both Nourishes and Harms Us
Hall, Kevin, PhD
Belluz, Julia
Hardcover
Fungi Decoded: Adventures in the Mycosphere--Encounters with Thirty Fungi
Bunyard, Britt A.
Paperback
Togetherness: Symbiosis and the Hidden Story of Life's Greatest Collaborations
Hooper, Rowan
Hardcover
Night Magic: Adventures Among Glowworms, Moon Gardens, and Other Marvels of the Dark
Henion, Leigh Ann
Hardcover
The Whole Truth: The Hidden Agendas Behind Decades of Health Misinformation--And What to Do about It
Campbell, T. Colin
Campbell, Nelson
Hardcover
The Big One: How We Must Prepare for Future Deadly Pandemics
Osterholm, Michael T.
Olshaker, Mark
Hardcover
Princeton Review AP Biology Premium Prep, 29th Edition: 6 Practice Tests + Digital Practice Online + Content Review
The Princeton Review
Paperback
Peterson Field Guide to Mushrooms of North America, Second Edition
McKnight Ward, Kirsten
McKnight, Karl B.
Rohrer, Joseph R.
Paperback
Cells at Work! Picture Book 1: The Great Bacteria vs. White Blood Cell Battle!
Makimura, Kumi
Hardcover
Bioinspired: How Nature Made Us Modern, Drives Today's Technological Innovation, and Inspires a Future Worth Inventing
Stier, Samuel Cord
Hardcover
A Year with the Seals: Unlocking the Secrets of the Sea's Most Charismatic and Controversial Creatures
Morris, Alix
Hardcover
VAX Facts: What to Consider Before Vaccinating at All Ages & Stages of Life
Thomas, Paul
Hoover, Deedee
Paperback
The Gut-Brain Paradox: Improve Your Mood, Clear Brain Fog, and Reverse Disease by Healing Your Microbiome
Gundry MD, Steven R.
Hardcover
Psilocybin: Magic Mushroom Grower's Guide: A Handbook for Psilocybin Enthusiasts
Oss, O. T.
Oeric, O. N.
Paperback
All That Remains: A Renowned Forensic Scientist on Death, Mortality, and Solving Crimes
Black, Sue
Paperback
The Aesthetic Revolution: Embracing Vanity and the Future of Fitness to Unlock Your Healthiest Self
Israetel, Mike
Hardcover
National Geographic the Photo Ark: One Man's Quest to Document the World's Animals
Sartore, Joel
Chadwick, Douglas H.
Hardcover
True Hallucinations: Being an Account of the Author's Extraordinary Adventures in the Devil's Paradis
McKenna, Terence
Paperback
Psilocybin Mushrooms: A Comprehensive Guide to Growing and Using Psychedelic Mushrooms
Madden, Michael
Paperback
Mushrooms of the Redwood Coast: A Comprehensive Guide to the Fungi of Coastal Northern California
Siegel, Noah
Schwarz, Christian
Paperback
Pull: How Gravity Shapes Your Body, Steadies the Mind, and Guides Our Health
Spiegel, Brennan
Hardcover
The Life Machines: How Taking Care of Your Mitochondria Can Transform Your Health
Mochly-Rosen, Daria
Rosen, Emanuel
Hardcover
Fungi of Temperate Europe: Keys to the Basidiomycotes
Frøslev, Tobias G.
Læssøe, Thomas
Petersen, Jens H.
Hardcover
The Little Book of Mushrooms: An Illustrated Guide to the Extraordinary Power of Mushrooms
Dorr, Alex
Hardcover
Entangled Life: How Fungi Make Our Worlds, Change Our Minds & Shape Our Futures
Sheldrake, Merlin
Hardcover
Princeton Review AP Biology Premium Prep, 28th Edition: 6 Practice Tests + Digital Practice Online + Content Review
The Princeton Review
Paperback
Mushrooming: An Illustrated Guide to the Fantastic, Delicious, Deadly, and Strange World of Fungi
Borsato, Diane
Hardcover
The Contagion Myth: Why Viruses (Including Coronavirus) Are Not the Cause of Disease
Cowan, Thomas S.
Fallon Morell, Sally
Hardcover
Mushrooms of Cascadia, Second Edition: An Illustrated Key to the Fungi of the Pacific Northwest
Beug, Michael
Paperback
From These Lands: Sharing Our Natural and Cultural Heritage
National Museum of Natural History
Hardcover
Human Errors: A Panorama of Our Glitches, from Pointless Bones to Broken Genes
Lents, Nathan H.
Paperback
The Ocean's Menagerie: How Earth's Strangest Creatures Reshape the Rules of Life
Harvell, Drew
Hardcover
How to Win Friends and Influence Fungi: Collected Quirks of Science, Tech, Engineering, and Math from Nerd Nite
Balakrishnan, Chris
Wasowski, Matt
Paperback
The Pale Blue Data Point: An Earth-Based Perspective on the Search for Alien Life
Willis, Jon
Hardcover
The Mycocultural Revolution: Transforming Our World with Mushrooms, Lichens, and Other Fungi
McCoy, Peter
Hardcover
Field Notes from a Fungi Forager: An Illustrated Journey Through the World of Pacific Northwest Mushrooms
Rodriguez, Ashley
Hardcover
Life in Sync: The Science of Internal Clocks and How We're Disrupting Them
Gander, Philippa
Hardcover
The Art and Science of Foodpairing: 10,000 Flavour Matches That Will Transform the Way You Eat
Langenbick, Johan
Coucquyt, Peter
Lahousse, Bernard
Hardcover
National Audubon Society Wildflowers of North America: The Complete Identification Reference to Wildflowers--With Full-Color Photographs; Updated Rang
National Audubon Society
Hardcover
Gut Check: Unleash the Power of Your Microbiome to Reverse Disease and Transform Your Mental, Physical, and Emotional Health
Gundry MD, Steven R.
Hardcover
Sea Wonders: The Octopus, the Cuttlefish, and the Squid
Tomasinelli, Francesco
Colombo, Marco
Hardcover
The Double Helix: A Personal Account of the Discovery of the Structure of DNA
Watson, James D.
Paperback
Understanding Ampicillin: Understanding Ampicillin: Indications, Administration, And Drug Interactions
Caldwell, Ethan
Paperback
Superconvergence: How the Genetics, Biotech, and AI Revolutions Will Transform Our Lives, Work, and World
Metzl, Jamie
Paperback
If the Ocean Has a Soul: A Marine Biologist's Pursuit of Truth Through Deep Waters of Faith and Science
Jordan, Rachel G.
Paperback
The Pfizer Papers: Pfizer's Crimes Against Humanity
The Warroom/Dailyclout Pfizer Documents Analysts
Hardcover
The Serengeti Rules: The Quest to Discover How Life Works and Why It Matters
Carroll, Sean B.
Paperback
Field Guide to Wild Mushrooms of Pennsylvania and the Mid-Atlantic: Revised and Expanded Edition
Russell, Bill
Paperback