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Germline Stem Cells: Methods and Protocols

Germline Stem Cells: Methods and Protocols

Paperback

Series: Methods in Molecular Biology, Book 2677

Biology

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ISBN10: 1071632612
ISBN13: 9781071632611
Publisher: Humana
Published: Jul 19 2024
Pages: 284
Language: English
This third edition volume expands on the previous editions with new discussion on the latest techniques and model systems used to study germline stem cells. The chapters in this book cover topics such as analysis of the C. elegans germline stem cell pool; live imaging of the Drosophila testis stem cell niche; standardization of single-cell RNA-sequencing analysis workflow to study Drosophila ovary; 3D analysis of the intact mouse ovary; and derivation and primordial germ cell induction of intermediate pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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Buszczak, Michael

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Biology