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Clinical Proteomics: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 1243
Anatomy & PhysiologyBiologyChemistry
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ISBN13: 9781493947294
Publisher: Humana
Published: Aug 23 2016
Pages: 278
Weight: 1.13
Height: 0.62 Width: 7.01 Depth: 10.00
Language: English
Part I: Sample Collection
1. Biological Sample Collection For Clinical Proteomics - Existing SOPs
Vasiliki Lygirou, Manousos Makridakis, and Antonia Vlahou
2. Targeting The Proteome Of Cellular Fractions: Focus on Secretome
Agnieszka Latosinska, Maria Frantzi, William Mullen, Antonia Vlahou and Manousos Makridakis
3. Preparation Of Urinary Exosomes: Methodological Issues For Clinical Proteomics
Marina Pitto, Samuele Corbetta, and Francesca Raimondo
4. Sample Treatment Methods Involving Combinatorial Peptide Ligand Libraries For Improved Proteomes Analyses
Pier Giorgio Righetti and Egisto Boschetti
5. Glycoprotein Enrichment Method Using A Selective Magnetic Nano-Probe Platform (MNP) Functionalized With Lectins
Marta Cova, Rui Oliveira-Silva, José Alexandre Ferreira, Rita Ferreira, Francisco Amado, Ana LuÃsa Daniel-Da-Silva, and Rui Vitorino
Part II: Technologies For Discovery and Validation
6. The Latest Advancements In Proteomic Two-Dimensional Gel Electrophoresis Analysis Applied To Biological Samples
Laura Santucci, Maurizio Bruschi, Gian Marco Ghiggeri, and Giovanni Candiano
7. 2DE Maps In The Discovery Of Human Autoimmune Kidney Diseases: The Case of Membranous Glomerulonephritis
Maurizio Bruschi, Laura Santucci, Gian Marco Ghiggeri, and Giovanni Candiano
8. MALDI-Imaging Mass Spectrometry on Tissues
Veronica Mainini, Maciej Lalowski, Athanasios Gotsopoulos, Vasiliki Bitsika, Marc Baumann, and Fulvio Magni
9. Laser Capture Microdissection Of Fluorescently Labeled Amyloid Plaques From Alzheimer's Disease Brain Tissue For Mass Spectrometric Analysis
Diana A.T. Nijholt, Christoph Stingl, and Theo M. Luider
10. Urine Sample Preparation And Fractionation For Global Proteome Profiling By LC-MS
Magali Court, Jérôme Garin, and Christophe D. Masselon
11. Methods In Capillary Electrophoresis Coupled To Mass Spectrometry For The Identification Of Clinical Proteomic/Peptidomic Biomarkers In Biofluids
Angelique Stalmach, Holger Husi, Khedidja Mosbahi, Amaya Albalat, William Mullen, and Harald Mischak
12. Quantification Of Proteins In Urine Samples Using Targeted Mass Spectrometry Methods
Nina Khristenko and Bruno Domon
Part III: Extracting Biological Relevant Biomarkers and Proceeding With Implementation
13. Statistical Issues In The Design And Planning Of Proteomic Profiling Experiments
David A. Cairns
14. Integrating Proteomics Profiling Data Sets - A Network Perspective
Akshay Bhat, Mohammed Dakna, and Harald Mischak
15. The European Medicines Agency Experience With Biomarker Qualification
Efthymios Manolis, Armin Koch, Dieter Deforce, and Spiros Vamvakas
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