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Cilia: Methods and Protocols

Cilia: Methods and Protocols

Hardcover

Series: Methods in Molecular Biology, Book 2725

Biology

ISBN10: 1071635069
ISBN13: 9781071635063
Publisher: Humana Pr
Published: Oct 20 2023
Pages: 278
Weight: 1.59
Height: 0.69 Width: 7.00 Depth: 10.00
Language: English

This volume covers the latest advancements in the study of ciliary complexity. Protocols cover genomic, proteomic, imaging, and functional analysis of different ciliated tissues and their wide applicability in cilia biology. Chapters in this book primarily focus on methods to study multiciliated cells, and discuss topics such as SARS-CoV-2 infections of human primary nasal multiciliated epithelial cells; expansion microscopy of ciliary proteins; live-imaging centriole amplification in mouse brain multiciliated cells; biophysical properties of cilia motility; and mucociliary transport device construction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cilia: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.

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Biology