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Cardiac Gene Therapy: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 1521
ISBN13: 9781493982431
Publisher: Humana
Published: Jul 6 2018
Pages: 355
Weight: 1.40
Height: 0.76 Width: 7.00 Depth: 10.00
Language: English
Part I: Overview
1. Current Methods in Cardiac Gene Therapy: Overview
Kiyotake Ishikawa and Roger J. Hajjar
Part II: Genetic Modification
2. Silencing Genes in the Heart
Henry Fechner, Roland Vetter, Jens Kurreck, and Wolfgang Poller
3. Generation of Efficient miRNA Inhibitors Using Tough Decoy Constructs
Jimeen Yoo, Roger J. Hajjar, and Dongtak Jeong
4. Efficient Genome Editing in Induced Pluripotent Stem Cells with Engineered Nucleases In Vitro
Vittavat Termglinchan, Timon Seeger, Caressa Chen, Joseph C. Wu, and Ioannis Karakikes
5. Direct Cardiac Reprogramming as a Novel Therapeutic Strategy for Treatment of Myocardial Infarction
Hong Ma, Li Wang, Jiandong Liu, and Li Qian
Part III: Gene Delivery Vectors
6. Production and Characterization of Vectors Based on the Cardiotropic AAV Serotype 9
Erik Kohlbrenner and Thomas Weber
7. Cell-Based Measurement of Neutralizing Antibodies Against Adeno-Associated Virus (AAV)
Andreas Jungmann, Oliver Müller, and Kleopatra Rapti
8. Synthesis of Modified mRNA for Myocardial Delivery
Jason Kondrat, Nishat Sultana, and Lior Zangi
9. Exosomes-Based Gene Therapy for MicroRNA Delivery
Prabhu Mathiyalagan and Susmita Sahoo
10. Lipidoid mRNA Nanoparticles for Myocardial Delivery in Rodents
Irene C. Turnbull, Ahmed A. Eltoukhy, Daniel G. Anderson, and Kevin D. Costa
Part IV: Gene Delivery Methods
11. Gene Transfer in Isolated Adult Cardiomyocytes
Kjetil Hodne, David B. Lipsett, and William E. Louch
12. Gene Transfer in Cardiomyocytes Derived from ES and iPS Cells
Francesca Stillitano, Ioannis Karakikes, and Roger J. Hajjar
13. Gene Transfer to Rodent Hearts In Vivo
Federica del Monte, Kiyotake Ishikawa, and Roger J. Hajjar
14. Ultrasound-Targeted Microbubble Destruction for Cardiac Gene Delivery
Shuyuan Chen and Paul A. Grayburn
15. A Needleless Liquid Jet Injection Delivery Approach for Cardiac Gene Therapy
Anthony S. Fargnoli, Michael G. Katz, and Charles R. Bridges
16. Cardiac Gene Delivery in Large Animal Models: Antegrade Techniques
Shin Watanabe, Lauren Leonardson, Roger J. Hajjar, and Kiyotake Ishikawa
17. Direct Myocardial Injection of Vectors
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