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Cancer Cell Biology: Methods and Protocols

Cancer Cell Biology: Methods and Protocols

Paperback

Series: Methods in Molecular Biology, Book 2508

Medical ReferenceBiology

ISBN10: 1071623788
ISBN13: 9781071623787
Publisher: Humana Pr
Published: Jun 25 2023
Pages: 379
Weight: 1.49
Height: 0.81 Width: 7.00 Depth: 10.00
Language: English

This volume provides detailed methods on the mechanisms of underlying cancer cell biology. Chapters guide readers through techniques for culturing cancer cell lines, xenografts, cryopreservation of tumor cells, analyzing the co-culture of breast cancer cells, protein secretion by ELISA, flow cytometry-based, multi-parametric immunofluorescence analysis, protein expression by western blot, analysis of surface protein levels, protein recycling by biotinylation assay, and proteomics analysis by liquid chromatography-mass spectrometry. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

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Christian, Sherri L.

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