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Basophils and Mast Cells: Methods and Protocols

Basophils and Mast Cells: Methods and Protocols

Paperback

Series: Methods in Molecular Biology, Book 2163

Medical ReferenceBiology

ISBN10: 1071606980
ISBN13: 9781071606988
Publisher: Humana Pr
Published: Aug 8 2021
Pages: 380
Weight: 1.53
Height: 0.82 Width: 7.00 Depth: 10.00
Language: English

This second edition provides updated and new chapters to build on and extend the strengths of the first edition. Chapters guide readers through basic biology of basophils, obtaining the cells by purification, culture of stem cells progenitors, peripheral CD34+ stem cell-derived mast cells, basophils from CD34+ progenitors, diagnostic applications, gene expression patterns in basophils, roles of basophils in different asthma phenotypes, knockout, and disease models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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