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Basic Cell Culture

Basic Cell Culture

Paperback

Series: Practical Approach, Book 254

Medical ReferenceBiologyBotany & Horticulture

ISBN10: 0199638535
ISBN13: 9780199638536
Publisher: OUP Oxford
Published: Mar 21 2002
Pages: 408
Weight: 1.62
Height: 0.90 Width: 7.46 Depth: 9.54
Language: English
The use of animal, including human, cell culture has expanded enormously during the last twenty years, with the new applications appearing all the time. This book guides the newcomer progressively through all those areas which are basic to the performance of cell culture. It will also prove useful to the experienced worker entering a new field or setting up a new laboratroy, and as a source of reference on basic techniques. The topics covered include setting up and equipping a cell culture laboratory, sterilization of fluids and equipment, culture media, basic culture technique and the maintenance of cell lines, primary culture and the isolation of new cell lines, specific cell types and their requirements, single cell cloning, quality control of cell lines and the prevention, detection, and cure of contamination.

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