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Base Editors: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 2606
BiologyGeneral ScienceGenetics
ISBN13: 9781071628782
Publisher: Humana
Published: Jan 3 2023
Pages: 235
Weight: 1.43
Height: 0.63 Width: 7.00 Depth: 10.00
Language: English
Part I: Overview of BEs, Diverse Variants of BEs, and Computational Tools for Bes
1. Introduction and Perspectives of DNA Base Editors
Beomjong Song and Sangsu Bae
2. Web-Based Computational Tools for Base Editors
Gue-Ho Hwang and Sangsu Bae
3. Prediction of Base Editing Efficiencies and Outcomes Using DeepABE and DeepCBE
Jinman Park and Hui Kwon Kim
4. Profiling Genome-Wide Specificity of dCpf1 Cytidine Base Editors Using Digenome-Seq
Daesik Kim
Part II: Application of BEs for Disease Modeling
5. Base Editing of Human Hematopoietic Stem Cells
Jing Zeng, Gabriele Casirati, My Anh Nguyen, Pietro Genovese, and Daniel E. Bauer
6. A/C Simultaneous Conversion Using the Dual Base Editor in Human Cells
Xiaohui Zhang, Yuting Guan, and Dali Li
7. Functional Analysis of Variants in BRCA1 Using CRISPR Base-Editors
Ji-Eun See and Yongsub Kim
8. Use of the Representative Base Editing Tool Target-AID to Introduce Pathogenic Mutations into Mice
Hiroki Sasaguri
9. Targeted Mutagenesis in Mice Using a Base Editor
Tae Yeong Jeong, Soo-Yeon Lim, Je Kyung Seong, and Kyoungmi Kim
Part III: Therapeutic Base Editing Strategies
10. Heterologous Expression and Purification of a CRISPR-Cas9-Based Adenine Base Editor
Seu-Na Lee, Hong-Su Jang, and Jae-Sung Woo
11. Delivering Base Editors In Vivo by Adeno-Associated Virus Vectors
Colin K.W. Lim, Angelo J. Miskalis, Pablo Perez-Pinera, and Thomas Gaj
12. The Delivery of ABE mRNA to the Adult Murine Liver by Lipid Nanoparticles (LNPs)
Dong Yang and Chun-Qing Song
13. Ex Vivo Base Editing Therapy with Chemically-Derived Hepatic Progenitors
Yohan Kim, Jaemin Jeong, and Dongho Choi
14. Application of Base Editor-Mediated Genome Editing in Mouse Retina
Jun Wu, Chang Sik Cho, Dong Hyun Jo, and Jeong Hun Kim
Part IV: Use of BEs in Plants and Bacteria
15. High-Throughput Base-Editing-Mediated Artificial Evolution Streamlines Trait Gene Identification in Rice
Fang Yan, Man Yu, Meixia Wang, and Huanbin Zhou
16. Heritable Virus-Induced Genome Editing (VIGE) in Nicotiana attenuate
Hyeonjin Kim, Youngbin Oh, Eunae Park, Moonyoung Kang, Yuri Choi, and Sang-Gyu Kim
17. Cytosine Base Editing in Bacteria
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