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Brain Development: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1082
Medical ReferenceAnthropologyCognition
ISBN13: 9781627036542
Publisher: Humana
Published: Sep 19 2013
Pages: 317
Weight: 1.63
Height: 0.94 Width: 7.35 Depth: 10.07
Language: English
Part I: Drosophila Protocols
1. Immunostaining of the Developing Embryonic and Larval Drosophila Brain
Danielle C. Diaper and Frank Hirth
2. Non-Fluorescent RNA In Situ-Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila
David Jussen and Rolf Urbach
3. Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI-Labeling
Karoline F. Kraft and Rolf Urbach
4. Flybow to Dissect Circuit Assembly in the Drosophila Brain
Nana Shimosako, Dafni Hadjieconomou, and Iris Salecker
5. Immunofluorescent Labeling of Neural Stem Cells in the Drosophila Optic Lobe
Benjamin Perruchoud and Boris Egger
6. Using MARCM to Study Drosophila Brain Development
Gudrun Viktorin
Part II: Other Arthropods
7. Dye Coupling and Immunostaining of Astrocyte-Like Glia Following Intracellular Injection of Fluorochromes in Brain Slices of the Grasshopper, Schistocerca gregaria
George Boyan and Yu Liu
Part III: Molluscs
8. Methods in Brain Development of Molluscs
Andreas Wanninger and Tim Wollesen
Part IV: Xenopus Protocols
9. In Situ Hybridization and Immunostaining of Xenopus Brain
Kai-li Liu, Xiu-mei Wang, Zi-long Li, and Ying Liu
10. Microinjection Manipulations in the Elucidation of Xenopus Brain Development
Cristine Smoczer, Lara Hooker, Saqib S. Sachani, and Michael J. Crawford
11. Morpholino Studies in Xenopus Brain Development
Jennifer E. Bestman and Hollis T. Cline
Part V: Zebrafish Protocols
12. Sensitive Whole-Mount Fluorescent In Situ Hybridization in Zebrafish Using Enhanced Tyramide Signal Amplification
Gilbert Lauter, Iris Söll, and Giselbert Hauptmann
13. Dynamic Neuroanatomy at Subcellular Resolution in the Zebrafish
Adèle Faucherre and Hernán López-Schier
14. Anatomical Dissection of Zebrafish Brain Development
Katherine J. Turner, Thomas G. Bracewell, and Thomas A. Hawkins
Part VI: Chicken Protocols
15. Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain
Richard P. Tucker and Qizhi Gong
16. Transplantation of Neural Tissue: Quail-Chick Chimeras
Andrea Streit and Claudio D. Stern
17. RNAi-Based Gene Silencing in Chicken Brain Development
Irwin Andermatt and Esther T. Stoeckli
Part VII: Mouse Protocols
18. Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development
Jinling Liu and Aimin Liu
19. In Utero Electroporation to Study Mouse Brain Development
Emilie Pacary and François Guillemot
20. The Cre/Lox System to Assess the Development of the Mouse Brain
Claudius F. Kratochwil and Filippo M. Rijli
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