Sign-In
Cart
Bioassays in Experimental and Preclinical Pharmacology
Paperback
Series: Springer Protocols Handbooks
ISBN13: 9781071612354
Publisher: Humana
Published: Feb 17 2022
Pages: 271
Weight: 1.09
Height: 0.60 Width: 7.00 Depth: 10.00
Language: English
1. Overview
2. Cell Culture Assays
2.1 Cell Cycle Assay: Propidium Iodide Marking
2.2 Apoptosis Assay: Annexin V/Propidium Iodide Marking
2.3 Cytotoxicity in Caco-2 cells by the Alamar blue method
2.4 Viability in Caco-2 cells stimulated with lipopolysaccharide (LPS)
2.5 Determination of Nitric oxide (NO) by in vitro experiment
2.6 Analysis of specific cytotoxicity against RAW 264.7 for determination anti-inflammatory activity in vitro
2.7 Scratching of Fibroblast cells (scratch assay)
2.8 Evaluation of the in vitro inflammatory response in AGS cells
3. Phytochemical Analysis
3.1 Preliminary phytochemical assays
3.2 Quantitative analysis of secondary metabolites
3.3 Thin Layer Chromatography Analysis
3.4 Analysis by High Performance Liquid Chromatography (HPLC)
3.5 Mass spectrometry
3.6 Electrospray Ionization Mass Spectrometry (ESI-MS) analysis3.7 Identification of phenolic compounds by HPLC-LC-ESI- MS/MS
3.8 Quantitative determination of total alkaloids content by HPLC3.9 Quantitative determination of total alkaloids content by HPLC
4. Preclinical Drug Dose Calculation
4.1 Conversion based on normalization of dose-to body surface area
4.2 Determination of animal equivalent dose5. Toxicology Studies (In Vitro and In Vivo)
5.1 Cytotoxicity evaluation using Alamar blue5.2 Micronucleus test
5.3 Comet test5.4 Effect on cell cycle
5.5 In vivo acute toxicity study
5.6 Subchronic toxicity (repeated doses)
6. Standard Procedure for Anesthesia in Preclinical Experiments
6.1 Planning and anesthetic care
6.2 Pre-Procedure
6.3 Experimental Procedure
7. General Considerations and Collection of Animal Blood
7.1 Vein blood collection
7.2 Tail vein blood collection
7.3 Cardiac puncture blood collection
7.4 Posterior vena cava
7.5 Orbital sinus8. Animal Experiments on the Cardiovascular System
8.1 Obtaining electrocardiogram (ECG) and blood pressure (BP) signals8.2 Myocardial ischemia induction
9. Animal Experiments on Ulcerative Colitis
9.1 Ulcerative colitis induced by 2,4,6-Trinitrobenzenesulfonic acid (TNBS)
9.2 Chronic ulcerative colitis induced by Dextran Sodium Sulfate (SSD) 2%9.3 Colitis with recurrence (Chronic)
10. Experiments on Anti-Bacterial Activities
10.1 Antibacterial activity by in vitro assays
10.2 Animal Experiments on antibacterial activity11. Experiments on Anti-Fungal Activities
11.1 Agar Disc Diffusion Method11.2 Minimum Inhibitory Concentration (MIC)
11.3 Sorbital test11.4 Neurospora crassa assay
11.5 Effect of Ergosterol on MIC
11.6 Test of Β1,3-D-Glucan Synthase (GS)
11.7
1 different editions
Also available
Bioassays in Experimental and Preclinical Pharmacology
Arunachalam, Karuppusamy
Sasidharan, Sreeja Puthanpura
Hardcover
Also from
Arunachalam, Karuppusamy
Bioassays in Experimental and Preclinical Pharmacology
Arunachalam, Karuppusamy
Sasidharan, Sreeja Puthanpura
Hardcover
Advanced Cell and Molecular Techniques: Protocols for in Vitro and in Vivo Studies
Arunachalam, Karuppusamy
Sreeja, Puthanpura Sasidharan
Hardcover
Advanced Cell and Molecular Techniques: Protocols for in Vitro and in Vivo Studies
Arunachalam, Karuppusamy
Sreeja, Puthanpura Sasidharan
Paperback
Advanced Protocols in Cancer Research: Translational Models for Antitumor and Anticancer Drug Discovery
Sreeja, Puthanpura Sasidharan
Anand, Krishnan
Arunachalam, Karuppusamy
Hardcover
Also in
Medical Reference
The Spirit Catches You and You Fall Down: A Hmong Child, Her American Doctors, and the Collision of Two Cultures
Fadiman, Anne
Paperback
7 Reasons to Believe in the Afterlife: A Doctor Reviews the Case for Consciousness After Death
Charbonier, Jean Jacques
Paperback
The Immune Mystery: A Doctor's Impassioned Quest to Solve the Puzzle of Autoimmune Disease
Dr Kåss, Anita
Jelstad, Jørgen
Hardcover
Holistic Reflexology: Essential Oils and Crystal Massage in Reflex Zone Therapy
Kliegel, Ewald
Paperback
The Boy Who Was Raised as a Dog: And Other Stories from a Child Psychiatrist's Notebook: What Traumatized Children Can Teach Us about Loss, Love, and
Perry, Bruce
Szalavitz, Maia
Paperback
Being Well (Even When You're Sick): Mindfulness Practices for People with Cancer and Other Serious Illnesses
Rosenbaum, Elana
Paperback
Overcoming Acute and Chronic Pain: Keys to Treatment Based on Your Emotional Type
Dibra, Sebhia Marie
Micozzi, Marc S.
Paperback
The Body Keeps the Score: Brain, Mind, and Body in the Healing of Trauma
Van Der Kolk, Bessel
Paperback
The Hunger Code: Resetting Your Body's Fat Thermostat in the Age of Ultra-Processed Food
Fung, Jason
Hardcover
Working The Roots: Over 400 Years of Traditional African American Healing
Lee, Michele Elizabeth
Paperback
Integrating a Palliative Approach Workbook 2nd Edition, Revised: Essentials For Personal Support workers
Murray, Katherine
Paperback
Unlearn Your Pain: The Science of Recovering from Chronic Pain, Fatigue, Anxiety, and Depression
Schubiner, Howard, MD
Hardcover
Jesus & Your Mental Health: Linking God's Word and Modern Science to Find Peace about Mental Health
Maxwell Lmft, Rebecca
Paperback
You've Been Pooping All Wrong: How to Make Your Bowel Movements a Joy
Pasricha, Trisha, MD MPH
Hardcover
THE DYSAUTONOMIA PROJECT Second Edition
Freeman, Kelly
Goldstein, David S.
Thompson, Charles R.
Paperback
Anatomy Coloring Book with 450+ Realistic Medical Illustrations with Quizzes for Each
McCann, Stephanie
Wise, Eric
Paperback
The Invincible Brain: The Clinically Proven Plan to Age-Proof Your Brain and Stay Sharp for Life
Fotuhi, Majid
Hardcover
Cialis & Men's Sexual Health: Understanding Tadalafil and Erectile Dysfunction
Stilton, John P.
Paperback
How to Forage for Mushrooms Without Dying: An Absolute Beginner's Guide to Identifying 29 Wild, Edible Mushrooms
Hyman, Frank
Paperback
The 36-Hour Day: A Family Guide to Caring for People with Alzheimer Disease, Other Dementias, and Memory Loss
Mace, Nancy L.
Rabins, Peter V.
Paperback
The Breast Advice: All You Need to Know about Breast Health, Screening, and Treatment
Port, Elisa
Hardcover
Plant Powered Plus: Activate the Power of Your Gut to Tame Inflammation and Reclaim Your Health
Bulsiewicz, Will
Hardcover
Period Repair Manual: Natural Treatment for Better Hormones and Better Periods
Briden Nd, Lara
Paperback
The Mindful Self-Compassion Workbook: A Proven Way to Accept Yourself, Build Inner Strength, and Thrive
Germer, Christopher
Neff, Kristin
Paperback
Mayo Clinic Guide to a Healthy, Happy Heart: Simple Steps to Achieving Long-Term Cardiovascular Health
Lara-Breitinger, Kyla M.
Lopez-Jimenez, Francisco
Lerman, Amir
Hardcover
Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition, Text Revision (Dsm-5-Tr(r))
American Psychiatric Association
Paperback
Think Less, Sleep More: From Panic & Perfectionism to Stress-Free Sleep
Romiszewski, Stephanie
Hardcover
Taking Charge of Adult ADHD: Proven Strategies to Succeed at Work, at Home, and in Relationships
Barkley, Russell A.
Paperback
Nolo's Guide to Social Security Disability: Getting & Keeping Your Benefits
Morton III, David A.
Paperback
All Creatures Great and Small: The Warm and Joyful Memoirs of the World's Most Beloved Animal Doctor
Herriot, James
Paperback
The Great Shadow: A History of How Sickness Shapes What We Do, Think, Believe, and Buy
Bauer, Susan Wise
Hardcover
The New Menopause: Navigating Your Path Through Hormonal Change with Purpose, Power, and Facts
Haver, Mary Claire
Hardcover
No One's Coming: The Rogue Heroes Our Government Turns to When There's Nowhere Else to Turn
Hazzard, Kevin
Hardcover
Medicare 101: From Your Initial Enrollment Period to Parts A, B, C, and D, an Essential Primer on the Government Healthcare Program
Lankford, Kimberly
Hardcover
El Cuerpo Lleva La Cuenta: Cerebro, Mente Y Cuerpo En La Superación del Trauma / The Body Keeps the Score
Van Der Kolk, Bessel
Paperback
The Ultimate Survival Medicine Guide: Emergency Preparedness for Any Disaster
Alton, Joseph
Alton, Amy
Paperback
Other Minds: The Octopus, the Sea, and the Deep Origins of Consciousness
Godfrey-Smith, Peter
Paperback
Why We Get Sick: The Hidden Epidemic at the Root of Most Chronic Disease--And How to Fight It
Bikman, Benjamin
Paperback
Cancer Is a Parasite: Kill It with the Safe, Over-The-Counter Antiparasitic Fenbendazole
Supple, William F., Jr.
Hardcover
Trauma and Recovery: The Aftermath of Violence---From Domestic Abuse to Political Terror
Herman, Judith Lewis
Paperback
Destiny of the Republic: A Tale of Madness, Medicine and the Murder of a President
Millard, Candice
Paperback
God's Hotel: A Doctor, a Hospital, and a Pilgrimage to the Heart of Medicine
Sweet, Victoria
Paperback
Harrison's Guide to Inpatient Medicine: Common Internal Medicine Diseases and Workups
Longo, Dan L.
Fauci, Anthony S.
Edwards, Mitchell
Hardcover
What Happened to You?: Conversations on Trauma, Resilience, and Healing
Winfrey, Oprah
Perry, Bruce D., M. D. PH. D.
Hardcover
Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition, Text Revision (Dsm-5-Tr(r))
American Psychiatric Association
Hardcover
Spiritual Portraits of the Energy Release Points: A Compendium of Acupuncture Point Messages Found Within the 12 Meridians and 8 Extraordinary Vessels
Gervais, Michele Marie
Paperback
Cialis Men's Usage Manual: Men's Long-Lasting Erection Pills: Quick-acting, Stay and Get Rock Hard, Unadulterated Mind-Blowing Climax
Petersson, Nicolas
Paperback
The Boy Who Was Raised as a Dog: And Other Stories from a Child Psychiatrist's Notebook--What Traumatized Children Can Teach Us about Loss, Love, and
Szalavitz, Maia
Perry, Bruce D.
Paperback
Change Your Brain, Change Your Pain: Breaking the Doom Loop to Heal Chronic Physical and Emotional Pain
Amen, Daniel G.
Hardcover
How to Forage for Medicinal Plants Without Dying: An Absolute Beginner's Guide to Identifying 32 Healing Wild Herbs
Ruiz, Brandon
Paperback
This Book May Cause Side Effects: The Curious and Dangerous Power of the Nocebo Effect, Placebo's Evil Twin
Pilcher, Helen
Hardcover
Medical Apartheid: The Dark History of Medical Experimentation on Black Americans from Colonial Times to the Present
Washington, Harriet A.
Paperback
The Spirit Catches You and You Fall Down: A Hmong Child, Her American Doctors, and the Collision of Two Cultures
Fadiman, Anne
Paperback
Forbidden Facts: Government Deceit & Suppression about Brain Damage from Childhood Vaccines
de Becker, Gavin
Hardcover
Desk Reference to the Diagnostic Criteria from Dsm-5-Tr(r)
American Psychiatric Association
Paperback
Stay Off My Kitchen Table: A Heart Surgeon's 4-Week Elimination Diet for Gut Repair, Metabolic Health, and Longevity
Lisec, Joshua
Ovadia, Philip
Paperback
The Obesity Code: Unlocking the Secrets of Weight Loss (Why Intermittent Fasting Is the Key to Controlling Your Weight)
Fung, Jason
Paperback
How to Change Your Mind: What the New Science of Psychedelics Teaches Us about Consciousness, Dying, Addiction, Depression, and Transcendence
Pollan, Michael
Paperback
The Doctors' Riot of 1788: Body Snatching, Bloodletting, and Anatomy in America
McPhee, Andy
Hardcover
Food Is Medicine: Healing Our Bodies, Nourishing Our Minds, and Transforming Our Food System
Mozaffarian, Dariush
Hardcover
Nursing School Entrance Exams Prep: Your All-In-One Guide to the Kaplan and Hesi Exams
Kaplan Nursing
Paperback
Cure Your Fatigue, Second Edition: The Root Cause and How to Fix It on Your Own
Robbins, Morley M.
Paperback
Desk Reference to the Diagnostic Criteria from Dsm-5-Tr(r)
American Psychiatric Association
Paperback
What Your Doctor Doesn't Know about Fibromyalgia: Why Doctors Can't or Won't Treat Chronic Pain
Rhoades, Patrick
Meilink, Linda
Paperback
Child Medication Fact Book for Psychiatric Practice, Second Edition
Tien, Elizabeth
Feder, Joshua D.
Puzantian, Talia
Paperback
Living Life to the Fullest with Ehlers-Danlos Syndrome: Guide to Living a Better Quality of Life While Having EDS
Muldowney Pt, Kathleen
Muldowney Pt, Kevin
Hardcover
Good Moms Have Scary Thoughts: A Healing Guide to the Secret Fears of New Mothers
Kleiman, Karen
Hardcover
Menopause: What Your Ob-GYN Wants You to Know
Obstetricians and Gynecologists, American College of
Paperback
Everything Is Tuberculosis: The History and Persistence of Our Deadliest Infection (Large Print Edition)
Green, John
Paperback
American Sirens: The Incredible Story of the Black Men Who Became America's First Paramedics
Hazzard, Kevin
Paperback
Pcos Is My Power: Understanding Pcos, Fixing Your Hormones, and Resetting Your Health
Ruth, Cory, Rdn
Paperback
Clinical Reasoning: Coaching the Struggling Medical Learner
Parsons, Andrew S.
Warburton, Karen M.
Paperback
The Yates Protocol: Five Simple Steps to Fix Your Blood Sugar and Reverse Type 2 Diabetes
Yates, Beverly
Hardcover
Science Under Siege: How to Fight the Five Most Powerful Forces That Threaten Our World
Hotez, Peter J.
Mann, Michael E.
Hardcover
Gut and Psychology Syndrome: Natural Treatment for Autism, Dyspraxia, A.D.D., Dyslexia, A.D.H.D., Depression, Schizophrenia, 2nd Edition
Campbell-McBride, Natasha, MD
Paperback
The Cure for Everything: The Epic Struggle for Public Health and a Radical Vision for Human Thriving
Williams, Michelle A.
Hardcover
Shoulder Pain? The Solution & Prevention: Fifth Edition, Revised & Expanded
Kirsch, John M.
Paperback
Hidden Epidemic: Silent Oral Infections Cause Most Heart Attacks and Breast Cancers
Levy, Jd
Paperback
Food Intelligence: The Science of How Food Both Nourishes and Harms Us
Hall, Kevin, PhD
Belluz, Julia
Hardcover
The Quantum Power of GLP-1 Peptides: Unlocking the Science that Leads to Lasting Weight Loss and Optimal Healthspan
Seeds, William A.
Paperback
Indoor Epidemic: 93% Inside Steals Sleep, Focus & Years-The 7% Outdoor Rx Restores Them
La Puma, John
Paperback
All the Names in the Bible: Comprehensive Index of Every Person and Place in Scripture
Thomas Nelson
Paperback
The Cortisol Cure: The Secret to Resetting Your Stress Response, Boosting Energy & Feeling Better Than Ever
Kinney, Erin
Hardcover
Curved Air: A Biography of Sickle Cell Anemia and the Quest to Cure the First Molecular Disease
Davies, Kevin
Hardcover
Fenbendazole & Ivermectin Protocols Handbook: A Definitive Guide on Treatment of Cancer for Patients and Caregivers
Willmott, Sebastian
Paperback
A Gentle Path Through the Twelve Steps: The Classic Guide for All People in the Process of Recovery
Carnes, Patrick J.
Paperback
The Unfragile Mind: A Physician's Call for Restoring Hope and Humanity to Mental Health Care
Francis, Gavin
Hardcover
The Other Side of the Straitjacket: A Daughter's Story of Mental Illness and Assisted Dying
Duncan, Alicia
Paperback
Current Medical Diagnosis & Treatment 2027
McQuaid, Kenneth R.
Papadakis, Maxine A.
Rabow, Michael W.
Hardcover