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Bacterial Chemosensing: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1729
ISBN13: 9781493975761
Publisher: Humana Pr
Published: Feb 11 2018
Pages: 389
Weight: 2.02
Height: 0.94 Width: 7.00 Depth: 10.00
Language: English
Part I: Introduction
1. The Diversity of Bacterial Chemosensing
Michael D. Manson
2. Transmembrane Signal Transduction in Bacterial Chemosensing
Michael D. Manson
Part II: Methods for Studying Bacterial Chemotaxis at the Population and Whole-Cell Levels
3. Two Spatial Chemotaxis Assays: The Nutrient-Depleted Chemotaxis Assay and the Agarose-Plug-Bridge Assay
Victoria Korolik and Karen M. Ottemann
4. Quantification of Bacterial Chemotaxis Responses at the Mouths of Hydrogel Capillaries
Benjamin A. Webb, Timofey Arapov, and Birgit Scharf
5. A Static Microfluidic Device for Investigating the Chemotaxis Response to Stable, Non-Linear Gradients
Nitesh Sule, Daniel Penarete-Acosta, Derek L. Englert, and Arul Jayaraman
6. Visualizing Chemoattraction of Planktonic Cells to a Biofilm
Sneha Jani
7. Labeling Bacterial Flagella with Fluorescent Dyes
Linda Turner and Howard C. BergPart III: In Vivo Analysis of Receptor Function
8. All-Codon Mutagenesis for Structure-Function Studies of Chemotaxis Signaling Proteins
Peter Ames and John S. Parkinson
9. Mutational Analysis of Binding Protein/Chemoreceptor Interactions
Andrew L. Seely
10. In Vitro Assay for Measuring Receptor-Kinase Activity in the Bacillus subtilis Chemotaxis Pathway
Hanna E. Walukiewicz, George W. Ordal, and Christopher V. Rao
11. FRET Analysis of the Chemotaxis Pathway Response
Anja Paulick and Victor Sourjik
12. Monitoring Two-Component Sensor Kinases with a Chemotaxis Signal Readout
Run-Zhi Lai and John S. Parkinson
13. Analyzing Protein Domain Interactions in Chemoreceptors by In Vivo PEGylation
Kylie J. Watts and Mark S. Johnson
14. Tuning Chemoreceptor Signaling by Positioning Aromatic Residues at the Lipid-Aqueous Interface
Rahmi Yusuf, Robert J. Lawrence, Lucy V. Eke, and Roger R. Draheim15. Analyzing Chemoreceptor Interactions In Vivo with the Tri-Functional Crosslinker TMEA
Claudia A. Studdert and Diego A. Massazza
Part IV: Cryo-EM Methods for Studying Chemoreceptor Structure
16. Use of Cryo-EM to Study the Structure of Chemoreceptor Arrays In Vivo
Wen Yang and Ariane Briegel
17. Visualizing Chemoreceptor Arrays in Bacterial Minicells by Cryo-Electron Tomography and Subtomogram Analysis
Zhuan Qin, Bo Hu, and Jun Liu
Part V: Monitoring the Intracellular Movement of Chemosensory Proteins
18. Bacterial Chemoreceptor Imaging at High Spatio-Temporal Resolution Using Photoconvertible Fluorescent Proteins
Jacopo Solari, Francois Anquez, Katharina Scherer, and Thomas S. Shimizu
19. Imaging of Single Dye-Labeled Chemotaxis Proteins in Live Bacteria Using Electroporation
Diana Di Paolo and Richard M. Berry
20. Fluorescence Anisotropy to Detect In Vivo Stimulus-Induced Changes in Chemoreceptor Packing
Vered Frank and Ady VakninPart VI: High-Throughput Methods for Screening Novel Chemoeffectors
21. Chemotaxis to Atypical Chemoattractants by Soil Bacteria
Rebecca E. Parales and Jayna L. Ditty
22. Screening Chemoreceptor-Ligand Interactions by High-Throughput Thermal-Shift Assays
Maximilian K.G. Ehrhardt, Suzanne L. Warring, and Monica L. Gerth
23. High-Throughput Screening to Identify Chemoreceptor Ligands
Mathilde FerAlso in
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