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Antibody Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 901
ISBN13: 9781617799303
Publisher: Springer Nature
Published: Jun 23 2012
Pages: 325
Weight: 1.70
Height: 1.00 Width: 7.20 Depth: 10.00
Language: English
1. Antigen Presentation for the Generation of Binding Molecules
Hilmar Ebersbach, Gabriele Proetzel, and Chonghui Zhang
2. Recombinant Antibodies and In Vitro Selection Technologies
C. Ronald Geyer, John McCafferty, Stefan Dübel, Andrew R.M. Bradbury, and Sachdev S. Sidhu
3. Phage Display
Konstantin Petropoulos
4. Generation of Human Fab Libraries for Phage Display
Christoph Rader
5. Selection of Human Fab Libraries by Phage Display
Christoph Rader
6. Ribosome Display
George Thom and Maria Groves
7. Hybridoma Technology for the Generation of Monoclonal Antibodies
Chonghui Zhang
8. The Application of Transgenic Mice for Therapeutic Antibody Discovery
E-Chiang Lee and Michael Owen
9. Production of Human or Humanized Antibodies in Mice
Brice Laffleur, Virginie Pascal, Christophe Sirac, and Michel Cogné
10. Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies
Elisabetta Traggiai
11. Kinetic Screening in the Antibody Development Process
Michael Schräml and Matthias Biehl
12. Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection
Michael Schräml and Leopold von Proff
13. Determination of Antibody Glycosylation by Mass Spectrometry
Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll
14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format
Jiahui Yang and Christoph Rader
15. PEGylation of Antibody Fragments for Half-Life Extension
Simona Jevsevar, Mateja Kusterle, and Maja Kenig
16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats
Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann
17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells
Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann
18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function
Robert F. Kelley and Y. Gloria Meng
19. Class-Specific Effector Functions of Therapeutic Antibodies
Virginie Pascal, Brice Laffleur, and Michel Cogné
Konstantin Petropoulos
4. Generation of Human Fab Libraries for Phage Display
Christoph Rader
5. Selection of Human Fab Libraries by Phage Display
Christoph Rader
6. Ribosome Display
George Thom and Maria Groves
7. Hybridoma Technology for the Generation of Monoclonal Antibodies
Chonghui Zhang
8. The Application of Transgenic Mice for Therapeutic Antibody Discovery
E-Chiang Lee and Michael Owen
9. Production of Human or Humanized Antibodies in Mice
Brice Laffleur, Virginie Pascal, Christophe Sirac, and Michel Cogné
10. Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies
Elisabetta Traggiai
11. Kinetic Screening in the Antibody Development Process
Michael Schräml and Matthias Biehl
12. Temperature
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