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Antibody Engineering: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1827
ISBN13: 9781493986477
Publisher: Humana Pr
Published: Sep 9 2018
Pages: 543
Weight: 2.60
Height: 1.19 Width: 7.00 Depth: 10.00
Language: English
Part I: In Silico Methods
1. Antibody Design and Humanization via In Silico Modeling
Vinodh B. Kurella and Reddy Gali
2. Antibody Affinity Maturation by Computational Design
Daisuke Kuroda and Kouhei Tsumoto
3. Use of IMGT(R) Databases and Tools for Antibody Engineering and Humanization
Marie-Paule Lefranc, François Ehrenmann , Sofia Kossida, Véronique Giudicelli, and Patrice Duroux
Part II: Generation of Diversity
4. Construction of Human Naïve Antibody Gene Libraries
Michela Pasello, Alessandra Mallano, Michela Flego, Silvia Zamboni, Anna Maria Giudice, and Katia Scotlandi
5. Construction of Synthetic Antibody Libraries
Déborah Caucheteur, Gautier Robin, Vincent Parez, and Pierre Martineau
6. Construction of Histidine-Enriched Shark IgNAR Variable Domain Antibody Libraries for the Isolation of pH-Sensitive vNAR Fragments
Doreen Könning, Steffen Hinz, Julius Grzeschik, Christian Schröter, Simon Krah, Stefan Zielonka, and Harald Kolmar
7. Display Technologies for Generation of Ig Single Variable Domains
Vladimir Bobkov, Bas van der Woning, and Hans de Haard
8. A Streamlined Approach for the Construction of Large Yeast Surface Display Fab Antibody Libraries
Simon Krah, Julius Grzeschik, Simon Rosowski, Ramona Gaa, Iris Willenbuecher, Deniz Demir, Lars Toleikis, Harald Kolmar, Stefan Becker, and Stefan Zielonka
Part III: Selections of Lead Candidate
9. Phage Display and Selections on Purified Antigens
Magali Colazet and Patrick Chames
10. Selection of Antibodies to Transiently Expressed Membrane Proteins Using Phage Display
Martina L. Jones, Stephen M. Mahler, and Sumukh Kumble
11. Selection of Antibody Fragments against Structured DNA by Phage Display
Mahdi Zeraati, Marcel E. Dinger, and Daniel Christ
12. Selection of Antibody Fragments by Yeast Display
Nathalie Scholler
13. Rapid Selection of High Affinity Antibody scFv Fragments Using Ribosome Display
Birgit Dreier and Andreas Plückthun
14. In Vitro Selection of Single Domain Antibody (VHH) Using cDNA Display
Naoto Nemoto, Shigefumi Kumachi, and Hidenao Arai
15. Sequencing and Affinity Determination of Antigen-Specific B Lymphocytes from Peripheral Blood
Peter Schofield, Rodrigo Vazquez-Lombardi, Mahmoud Abdelatti, Damien Nevoltris, Christopher C. Goodnow, Daniel Christ, and Joanne H. Reed
Part IV: Production of Rec
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