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Antibody Engineering: Methods and Protocols, Second Edition
Hardcover
Series: Methods in Molecular Biology, Book 907
ISBN13: 9781617799730
Publisher: Springer Nature
Published: Aug 21 2012
Pages: 734
Weight: 3.35
Height: 1.90 Width: 7.35 Depth: 10.22
Language: English
Part I. In silico Methods
1. Use of IMGT(R) Databases and Tools for Antibody Engineering and Humanization
Marie-Paule Lefranc, François Ehrenmann, Chantal Ginestoux, Véronique Giudicelli, and Patrice Duroux
2. Computer-Assisted Modeling of Antibody Variable Domains
Oscar H. P. Ramos
Part II. Generation of Diversity
3. Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells
Lars Toleikis and André Frenzel
4. Human in Cell scFv Library from Infiltrating B Cell
Sylvie Peraldi-Roux
5. Construction of Human Naive Antibody Gene Libraries
Michael Hust, André Frenzel, Torsten Meyer, Thomas Schirrmann, and Stefan Dübel
6. Synthetic Customized scFv Libraries
Gautier Robin and Pierre Martineau
7. Selection of Stable scFv Antibodies by Phage Display
Eeva-Christine Brockmann
8. Generation of Single Domain Antibody Fragments Derived from Camelids and Generation of Manifold Constructs
Cécile Vincke, Carlos Gutiérez, Ulrich Wernery, Nick Devoogdt, Gholamreza Hassanzadeh-Ghassabeh, and Serge Muyldermans
9. Generation and Isolation of Target Specific Single Domain Antibodies from Shark Immune Repertoires
Mischa Roland Müller, Ronan O'Dwyer, Marina Kovaleva, Fiona Rudkin, Helen Dooley, and Caroline Jane Barelle
10. Generation of Human Single Domain Antibody Repertoires by Kunkel Mutagenesis
Kip Dudgeon, Romain Rouet, Kristoffer Famm, and Daniel Christ
Part IV. Selections of Lead Candidate
11. Phage Display and Selections on Purified Antigens
Julie Matz and Patrick Chames
12. Phage Display and Selections on Cells
Klervi Even-Desrumeaux and Patrick Chames
13. Humanization by CDR Grafting and Specificity-Determining Residue Grafting
Jin Hong Kim and Hyo Jeong Hong
14. Humanization by Guided Selections
Sang Jick Kim and Hyo Jeong Hong
15. Selection of Antibody Fragments by Yeast Display
Nathalie Scholler
16. Evolution of Antibodies in vitro by Ribosome Display
Bryan M. Edwards and Mingyue He
17. Mammalian Cell Display of Full Length IgG
Chen Zhou and Wenyan (David) Shen
Part V. Production of Recombinant Antibodies
18. Production of Antibody Fragments in Escherichia coli
Tomohisa Katsuda, Hiroyuki Sonoda, and Hideki Yamaji
19. Production of Antibody Derivatives in the Methylotrophic Yeast Pichia pastoris
Steve Schoonooghe, Jannick Leoen, and Jurgen Haustraete
20. Monoclonal Antibody Expression in Mammalian Cells
Richard (Yi) Zhang and Wenyan (David) Shen
21. Production of Recombinant Antibodies in Drosophila melanogaster S2 Cells
Daniel X. Johansson, Thomas Krey, and Oskar Andersson
22. Production of Antibody Fragments Using the Baculovirus-Insect Cell System
Takanori Furuta, Takafumi Ogawa, and Hideki Yamaji
23. Transient and Stable Expression of Antibodies in Nicotiana Species
J. Christopher Hall, Michael D. McLean and Freydoun Garabagi
Part VI. Variable Domain Optimization
24. Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance
Stephen Hearty, Paul Leonard, and Richard O'Kennedy
25. Affinity Determination of Biotinylated Antibodies by Flow Cytometry
Klervi Even-Desrumeaux and Patrick Chames
26. Affinity Maturation of Antibodies: Optimized Methods to Generate High Quality scFv Libraries and Isolate IgG Candidates by High Throughput Screenin
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