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Astrocytes: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1938
ISBN13: 9781493990672
Publisher: Humana
Published: Jan 8 2019
Pages: 295
Weight: 1.66
Height: 0.75 Width: 7.00 Depth: 10.00
Language: English
Part I: Astrocyte Biology: Overviews and Perspectives
1. The Astrocyte-Neuron Interface: An Overview on Molecular and Cellular Dynamics Controlling Formation and Maintenance of the Tripartite Synapse
Uzma Hasan and Sandeep K. Singh
2. Role of Astrocytes in the Neurogenic Niches
Julia Schneider, Julian Karpf, and Ruth Beckervordersandforth
Part II: Isolation of Astrocytes from Animal Models
3. Isolation and Culture of Astrocytes from Postnatal and Adult Mouse Brains
Yingbo He, Natalie Taylor, and Anindya Bhattacharya
4. Fluorescence-Activated Cell Sorting-Based Isolation and Characterization of Neural Stem Cells from the Adult Zebrafish Telencephalon
Rossella Di Giaimo, Sven Aschenbroich, and Jovica Ninkovic
Part III: Astrocyte Morphology and Functions: Emerging Methods for Their Characterizations In Vivo, In Situ, and In Vitro
5. A Method to Visualize the Nanoscopic Morphology of Astrocytes In Vitro and In Situ
Janosch P. Heller and Dmitri A. Rusakov
6. A Specialized Method to Resolve Fine 3D Features of Astrocytes in Non-Human Primate (Marmoset, Callithrix Jacchus) and Human Fixed Brain Samples
Gaël Quesseveur, Aymeric Fouquier d'Hérouël, Keith K. Murai, and David S. Bouvier
7. Cisterna Magna Injection in Rats to Study Glymphatic Function
Marta Ramos, Nicholas Burdon Bechet, Roberta Battistella, Chiara Pavan, Anna L.R. Xavier, Maiken Nedergaard, and Iben Lundgaard
8. Preparing the Astrocyte Perivascular Endfeet Transcriptome to Investigate Astrocyte Molecular Regulations at the Brain Vascular Interface
Anne-Cécile Boulay, Noémie Mazaré, Bruno Saubaméa, and Martine Cohen-Salmon
9. Monitoring Interneuron-Astrocyte Signaling and Its Consequences on Synaptic Transmission
Sara Mederos and Gertrudis Perea
10. Astrocytes and Circadian Rhythms: An Emerging Astrocyte-Neuron Synergy in the Timekeeping System
Olga Barca Mayo, Luca Berdondini, and Davide De Pietri Tonelli
11. In Vitro Engulfment Assay to Measure Phagocytic Activity of Astrocytes Using Synaptosomes
Youkyeong Gloria Byun and Won-Suk Chung
12. Quantifying Compartment-Specific Protein Translocation in Astrocytes by Object-Oriented Image Analysis: Mitochondrial Translocation of PKCδ
Kiavasch M.N. Farid and Amin Derouiche
13. Imaging of Local and Global Sodium Signals in Astrocytes
Niklas J. Gerkau, Karl W. Kafitz, and Christine R. Rose
14. Detection of Protein Uptake in In Vitro Cultured Astrocytes Exemplified by the Uptake of the Serine Protease, Tissue Plasminogen Activator
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