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Antimicrobial Activity & Anticancer activity from Marine Bacterium

Antimicrobial Activity & Anticancer activity from Marine Bacterium

Paperback

Biology

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ISBN10: 3659642975
ISBN13: 9783659642975
Publisher: Lap Lambert Academic Pub
Published: Jan 6 2015
Pages: 64
Weight: 0.23
Height: 0.15 Width: 6.00 Depth: 9.00
Language: English
The marine water samples were collected from the Kilakarai deep sea, the bacterial colonies were isolated and the dominated colonies were picked out and their morphological characterization was studied. From the biochemical characterization the isolated colony results as Bacillus sp. The antimicrobial potential were analysed and the resulted Fraction I, Fraction II, Fraction III shows the greater effect when against with the clinical pathogens. Staphylococcus aureus, Pseudomonas sp, Fraction III shows greater zone of inhibition in various concentration of (25 μl, 50 μl, 75 μl, 100 μl). The higher concentration of 100 μl of sample shows the greater results. The high concentration of the 100 μl of the crude sample exhibit higher zone of inhibition against the Staphylococcus sp (14mm), Pseudomonas (13mm), The results suggested that the fraction-III warranted further purification in order to unveil the active components of Pseudomonas sp. From the curve of elution in DEAE Sepharose Fast Flow chromatography Three A280 nm peak fractions, named G-1, G-2, and G-3 were collected respectively.

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