Open Daily: 10am - 10pm | Alley door pickup 10am - 7pm 3038 Hennepin Ave Minneapolis, MN
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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
A Practical Guide to Influenza Virus Isolation and Identification

A Practical Guide to Influenza Virus Isolation and Identification

Khan, Anish
Boora, Sanjit
Kaushik, Samander

Paperback

Biology

ISBN10: 6209309305
ISBN13: 9786209309304
Publisher: LAP Lambert Academic Publishing
Published: Dec 23 2025
Pages: 52
Weight: 0.18
Height: 0.12 Width: 6.00 Depth: 9.00
Language: English
This practical guide details the essential methodologies for the isolation, identification, and quantification of Influenza A viruses, specifically focusing on the H1N1pdm09 strain using MDCK cell culture systems. It provides a comprehensive workflow that includes standardized protocols for the maintenance and subculturing of MDCK cells, viral propagation techniques, and molecular confirmation via RT-PCR targeting the HA gene. Furthermore, the text offers in-depth instruction on viral quantification methods, employing Hemagglutination (HA) assays to determine total viral titers and TCID50 assays using the Reed-Muench method for calculating infectious titers. By combining theoretical background with rigorous experimental data and step-by-step procedural flowcharts, this work serves as a vital resource for researchers and laboratory professionals establishing robust platforms for influenza virus characterization and antiviral drug discovery.

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