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Engenharia Genética: Da teoria à prática
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ISBN10: 6207978226
ISBN13: 9786207978229
Publisher: Edicoes Nosso Conhecimento
Published: Aug 22 2024
Pages: 276
Weight: 0.90
Height: 0.62 Width: 6.00 Depth: 9.00
Language: Portuguese
ISBN13: 9786207978229
Publisher: Edicoes Nosso Conhecimento
Published: Aug 22 2024
Pages: 276
Weight: 0.90
Height: 0.62 Width: 6.00 Depth: 9.00
Language: Portuguese
Embarque numa viagem ao mundo de ponta da engenharia genética com este livro abrangente! O livro aprofunda as principais técnicas laboratoriais que transformam a genética, desde o isolamento de ADN celular e cromossómico até ao domÃnio do isolamento de genes com métodos como a absorção de sÃlica e Chelex.Conceba sondas de ADN e explore experiências avançadas como a Bergs Terminal Transferase e Boyer-Cohen-Chang. Adquira experiência na preparação de células competentes para uma absorção eficiente do ADN e compreenda a transformação bacteriana através de técnicas de electroporação e mediadas por CaCl2. Explore processos dinâmicos como SDS-PAGE para análise de proteÃnas e seleção de colónias brancas azuis utilizando X-Gal/IPTG. Conheça as complexidades do genoma, a hibridação in situ, a amplificação do ADN em tempo real e a RT-PCR, as técnicas de blotting e a sequenciação Sanger e Maxam-Gilbert.'Engenharia Genética: Da teoria à prática é um recurso essencial para estudantes, investigadores e profissionais ansiosos por aproveitar o potencial da engenharia genética. Cada capÃtulo abre uma porta de entrada para possibilidades extraordinárias na intersecção da ciência e da inovação, tornando este livro imprescindÃvel para todos os apaixonados pela fronteira genética.
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