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Le génie génétique: De la théorie à la pratique
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ISBN10: 6207978250
ISBN13: 9786207978250
Publisher: Editions Notre Savoir
Published: Aug 22 2024
Pages: 280
Weight: 0.91
Height: 0.63 Width: 6.00 Depth: 9.00
Language: French
ISBN13: 9786207978250
Publisher: Editions Notre Savoir
Published: Aug 22 2024
Pages: 280
Weight: 0.91
Height: 0.63 Width: 6.00 Depth: 9.00
Language: French
Embarquez pour un voyage dans le monde de pointe du génie génétique avec ce livre complet ! Ce livre aborde les principales techniques de laboratoire qui transforment la génétique, de l'isolement de l'ADN cellulaire et chromosomique à la maîtrise de l'isolement des gènes à l'aide de méthodes telles que l'absorption de la silice et le Chelex.Concevez des sondes ADN et explorez des expériences avancées telles que la transférase terminale de Bergs et Boyer-Cohen-Chang. Acquérir une expertise dans la préparation de cellules compétentes pour une absorption efficace de l'ADN, et comprendre la transformation bactérienne via l'électroporation et les techniques médiées par le CaCl2. Explorer des processus dynamiques tels que le SDS-PAGE pour l'analyse des protéines et la sélection de colonies bleu-blanc en utilisant le X-Gal/IPTG. Découvrez les complexités du génome, l'hybridation in situ, l'amplification de l'ADN en temps réel et la RT-PCR, les techniques de blotting et le séquençage Sanger et Maxam-Gilbert.Le génie génétique: De la théorie à la pratique est une ressource essentielle pour les étudiants, les chercheurs et les professionnels désireux d'exploiter le potentiel du génie génétique. Chaque chapitre ouvre la voie à des possibilités extraordinaires à l'intersection de la science et de l'innovation, ce qui en fait un ouvrage indispensable pour tous ceux qui sont passionnés par les frontières de la génétique.
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