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Strategie inżynierii genetycznej
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ISBN10: 6630131444
ISBN13: 9786630131444
Publisher: Wydawnictwo Nasza Wiedza
Published: Jun 17 2026
Pages: 112
Weight: 0.35
Height: 0.27 Width: 6.00 Depth: 9.00
Language: Polish
ISBN13: 9786630131444
Publisher: Wydawnictwo Nasza Wiedza
Published: Jun 17 2026
Pages: 112
Weight: 0.35
Height: 0.27 Width: 6.00 Depth: 9.00
Language: Polish
Umiejętności i wiedza z zakresu inżynierii genetycznej lub szlaków metabolicznych pozwalają na wykorzystanie standardowych zasobów laboratoryjnych oraz informacji z publicznych baz danych do osiągania niezwyklych rezultatów poprzez manipulowanie na poziomie molekularnym. Techniki omówione w niniejszym artykule mają charakter uniwersalny i mają zastosowanie do wszelkich szlaków biochemicznych związanych ze środowiskiem komórkowym lub subkomórkowym, które mogą odgrywac kluczową rolę w zależności od swoich wlaściwości. Zostaly one przedstawione krok po kroku i logicznie wywnioskowane na przykladzie szlaku związanego z pantotenianem, który stanowi przyklad zlożoności, z jaką można się spotkac podczas projektowania eksperymentów. Metody te polegają na zwiększaniu lub zmniejszaniu przeplywu metabolicznego w danym węźle lub na kierowaniu nadmierną/niedostateczną produkcją metabolitu, a także na ilościowym określeniu efektu za pomocą wydajnych i niedrogich technik bioanalizy oraz modeli statystycznych. Książka ta stanowi przewodnik dla osób pragnących opanowac sztukę prowadzenia badań naukowych w warunkach ograniczonego budżetu. Dla osób pragnących poglębic swoją wiedzę na temat kontroli strumienia metabolicznego w celu lepszego zrozumienia funkcji loci genomowych omówione strategie i techniki są niezbędne do osiągnięcia korzyści komercyjnych. Książka może slużyc jako material referencyjny na studiach wyższych na poziomie zaawansowanym.
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