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Stratégies de génie génétique
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ISBN10: 6630126327
ISBN13: 9786630126327
Publisher: Editions Notre Savoir
Published: Jun 22 2026
Pages: 112
Weight: 0.35
Height: 0.27 Width: 6.00 Depth: 9.00
Language: French
ISBN13: 9786630126327
Publisher: Editions Notre Savoir
Published: Jun 22 2026
Pages: 112
Weight: 0.35
Height: 0.27 Width: 6.00 Depth: 9.00
Language: French
Les compétences et les connaissances en matière d'ingénierie génétique ou des voies métaboliques permettent de tirer le meilleur parti des ressources de laboratoire standard et des informations issues des bases de données publiques en intervenant au niveau moléculaire. Les techniques abordées dans cet article sont celles qui s'appliquent de manière générique à toutes les voies biochimiques liées à l'environnement cellulaire ou subcellulaire et qui peuvent jouer un rôle central en fonction de leurs caractéristiques. Elles sont présentées étape par étape et déduites de manière logique à partir de l'exemple de la voie du pantothénate, qui illustre bien l'ampleur de la complexité à laquelle on peut être confronté lors de la conception d'expériences. Ces méthodes consistent à réguler à la hausse ou à la baisse le flux métabolique d'un noeud, ou à orienter la surproduction ou la sous-production d'un métabolite, puis à quantifier l'effet à l'aide de techniques de bioessais efficaces et peu coûteuses ainsi que de modèles statistiques. Cet ouvrage sert de guide à ceux qui aspirent à maîtriser l'art de mener des travaux de recherche dans un environnement à budget limité. Pour ceux qui souhaitent approfondir leur compréhension du contrôle des flux métaboliques afin de mieux cerner la fonction des loci génomiques, les stratégies et techniques abordées sont indispensables pour obtenir des retombées commerciales. Cet ouvrage peut servir de référence pédagogique dans le cadre des études univ
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