Open Daily: 10am - 10pm | Alley door pickup 10am - 7pm 3038 Hennepin Ave Minneapolis, MN
612-822-4611

  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Heavy Metal Toxicity in Bioremediation: Microbial Cultures and Microscopy

Heavy Metal Toxicity in Bioremediation: Microbial Cultures and Microscopy

Goodbody, Jason B.

Paperback

Technology & EngineeringEnvironmental StudiesGeneral Education

ISBN10: 1288282729
ISBN13: 9781288282722
Publisher: Biblioscholar
Published: Nov 12 2012
Pages: 124
Weight: 0.41
Height: 0.26 Width: 6.14 Depth: 9.21
Language: English

This research employed a variety of microscopy and spread plating techniques to observe the effects of heavy metal treatments on a toluene-selected bacterial population. Microbial colonies were cultured on spread plates and the resulting numbers were compared to respiration data. The mechanisms of reproduction were demonstrated to be more sensitive to metal treatments than were the mechanisms of respiration. Phase contrast, Gram stain, fluorescent microscopy, were used to compare and document a wide variety of bacteria resulting from different metal treatments as well as from environmental changes within the source bioreactor. The removal of sensitive bacteria and the selection of metal tolerant species resulting from metal treatments was observed. Species that were initially unobserved within the bioreactor appeared to dominate when competing types of bacteria were removed and more agreeable environmental conditions were present. The use of fluorescent stains to differentiate between live and dead bacteria when treated with heavy metals proved to be impractical as the bacteria exhibited auto-fluorescence. Such new findings, however, did aid in the characterization of different types of bacteria and offered new techniques for potential heavy metal toxicity measurements as well as differentiation methods.

Also in

General Education

View All