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CRISPR / Cas9 - Unlocking the Potential of Genome Editing: Mechanisms - Engineering - Delivery - Therapeutics - Bench Protocols
Paperback
Series: Crispr/Cas: The 100
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ISBN13: 9798199972895
Publisher: Independently Published
Published: Jun 4 2026
Pages: 416
Weight: 2.93
Height: 1.08 Width: 8.50 Depth: 11.00
Language: English
Publisher: Independently Published
Published: Jun 4 2026
Pages: 416
Weight: 2.93
Height: 1.08 Width: 8.50 Depth: 11.00
Language: English
Preface
Few technologies in the history of the life sciences have traversed the distance from esoteric curiosity to clinical reality as rapidly as CRISPR-Cas genome editing. In little more than a decade, a set of repetitive sequences once dismissed as genomic oddities has been reconceived as an adaptive immune system, repurposed as a programmable nuclease, engineered into a versatile platform for rewriting genetic information, and translated into licensed medicines. The pace of this transformation has been so extraordinary that even specialists struggle to maintain a synthetic view of the field. This volume is an attempt to provide that synthesis: a rigorous, integrated account of the molecular biology, protein engineering, delivery science, and translational application of CRISPR-Cas technologies, written for those who intend not merely to read about genome editing but to practise it.
Few technologies in the history of the life sciences have traversed the distance from esoteric curiosity to clinical reality as rapidly as CRISPR-Cas genome editing. In little more than a decade, a set of repetitive sequences once dismissed as genomic oddities has been reconceived as an adaptive immune system, repurposed as a programmable nuclease, engineered into a versatile platform for rewriting genetic information, and translated into licensed medicines. The pace of this transformation has been so extraordinary that even specialists struggle to maintain a synthetic view of the field. This volume is an attempt to provide that synthesis: a rigorous, integrated account of the molecular biology, protein engineering, delivery science, and translational application of CRISPR-Cas technologies, written for those who intend not merely to read about genome editing but to practise it.
The intended readership is deliberately broad within the research community. Graduate students entering the field will find the conceptual scaffolding required to read the primary literature critically. Postdoctoral scientists and principal investigators migrating from adjacent disciplines will find the practical detail necessary to design experiments and interpret outcomes. Established molecular biologists will find a consolidated reference that connects mechanism to method and method to application.
The organisation of the book follows the internal logic of the technology rather than its chronology. The opening chapters establish the biological foundations: the natural history of CRISPR-Cas immunity, the molecular architecture and catalytic mechanism of the Cas9 enzyme, the remarkable diversity of CRISPR-Cas systems, and the cellular DNA-repair pathways that ultimately determine editing outcomes. The middle chapters treat the engineering of the toolbox: high-fidelity and PAM-flexible nuclease variants, the expanding repertoire of Cas effectors beyond Cas9, and the precision platforms of base editing and prime editing that rewrite sequence without reliance on double-strand breaks. The later chapters address the practical and translational dimensions of the field: the delivery technologies that carry editing reagents to their targets, the design of guide RNAs and the genome-wide analysis of off-target activity, the use of CRISPR screening in functional genomics, and the application of editing in therapeutics, agriculture, diagnostics, and microbial engineering.
A distinguishing feature of this volume is the integration of detailed laboratory protocols within the conceptual chapters rather than their relegation to an appendix. Reagent compositions, concentrations, incubation temperatures, centrifugation forces, timings, and troubleshooting guidance are presented alongside the principles that motivate them, on the conviction that method and mechanism are most durably learned together. These protocols are representative starting points, validated by widespread use in the literature, but they are not substitutes for empirical optimisation.
Finally, this book is is unreservedly an account of one of the most powerful technologies ever devised, but it is also a sober examination of that technology's limits and hazards. The narrative of triumph that surrounds genome editing is genuine, yet it is incomplete without the counter-narrative: the persistence of off-target and on-target genotoxicity, the chromosomal rearrangements and unintended large deletions that high-resolution assays continue to reveal, the clinical setbacks and at least one treatment-related death that have punctuated the therapeutic programme, and the profound disparity between the populations who bear the greatest burden of genetic disease and those who can access a therapy priced in the millions.
The organisation of the book follows the internal logic of the technology rather than its chronology. The opening chapters establish the biological foundations: the natural history of CRISPR-Cas immunity, the molecular architecture and catalytic mechanism of the Cas9 enzyme, the remarkable diversity of CRISPR-Cas systems, and the cellular DNA-repair pathways that ultimately determine editing outcomes. The middle chapters treat the engineering of the toolbox: high-fidelity and PAM-flexible nuclease variants, the expanding repertoire of Cas effectors beyond Cas9, and the precision platforms of base editing and prime editing that rewrite sequence without reliance on double-strand breaks. The later chapters address the practical and translational dimensions of the field: the delivery technologies that carry editing reagents to their targets, the design of guide RNAs and the genome-wide analysis of off-target activity, the use of CRISPR screening in functional genomics, and the application of editing in therapeutics, agriculture, diagnostics, and microbial engineering.
A distinguishing feature of this volume is the integration of detailed laboratory protocols within the conceptual chapters rather than their relegation to an appendix. Reagent compositions, concentrations, incubation temperatures, centrifugation forces, timings, and troubleshooting guidance are presented alongside the principles that motivate them, on the conviction that method and mechanism are most durably learned together. These protocols are representative starting points, validated by widespread use in the literature, but they are not substitutes for empirical optimisation.
Finally, this book is is unreservedly an account of one of the most powerful technologies ever devised, but it is also a sober examination of that technology's limits and hazards. The narrative of triumph that surrounds genome editing is genuine, yet it is incomplete without the counter-narrative: the persistence of off-target and on-target genotoxicity, the chromosomal rearrangements and unintended large deletions that high-resolution assays continue to reveal, the clinical setbacks and at least one treatment-related death that have punctuated the therapeutic programme, and the profound disparity between the populations who bear the greatest burden of genetic disease and those who can access a therapy priced in the millions.
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