Open Daily: 10am - 10pm | Alley door pickup 10am - 7pm 3038 Hennepin Ave Minneapolis, MN
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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Recombinant Protein Expression in Mammalian Cells: Methods and Protocols

Recombinant Protein Expression in Mammalian Cells: Methods and Protocols

Hacker, David L.

Hardcover

Series: Methods in Molecular Biology, Book 1850

BiologyChemistry

ISBN10: 1493987291
ISBN13: 9781493987290
Publisher: Humana
Published: Sep 22 2018
Pages: 311
Weight: 1.71
Height: 0.75 Width: 7.00 Depth: 10.00
Language: English

This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host; however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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