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RNA and DNA Editing: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 718
ChemistryGeneral ScienceGenetics
ISBN13: 9781493958214
Publisher: Humana Pr
Published: Aug 23 2016
Pages: 275
Weight: 1.12
Height: 0.61 Width: 7.00 Depth: 10.00
Language: English
Part I: Uracil Insertion/Deletion RNA Editing in Mitochondrion of Trypanosoma brucei
1. Three-Dimensional Reconstruction of Trypanosoma brucei Editosomes Using Single-Particle Electron Microscopy
H. Ulrich Göringer, Holger Stark, Cordula Böhm, Bjoern Sander, and Monika M. Golas
2. iCODA: RNAi-Based Inducible Knock-In System in Trypanosoma brucei
Gene-Errol Ringpis, Richard H. Lathrop, and Ruslan Aphasizhev
Part II: Adenosine to Inosine RNA Editing
3. Perturbing A-to-I RNA Editing Using Genetics and Homologous Recombination
Cynthia J. Staber, Selena Gell, James E.C. Jepson, and Robert A. Reenan
4. Laser Microdissection and Pressure Catapulting of Single Human Motor Neurons for RNA Editing Analysis
Hui Sun, Mary A. O'Connell, Valerie Mann, Brendon Noble, and Liam P. Keegan
5. Biochemical Identification of A-to-I RNA Editing sites by the Inosine Chemical Erasing (ICE) Method
Masayuki Sakurai and Tsutomu Suzuki
Part III: Cytidine to Uracil RNA and DNA Editing
6. Identifying mRNA Editing Deaminase Targets by RNA-Seq
Brad R. Rosenberg, Scott Dewell, and F. Nina Papavasiliou
7. Mouse and Other Rodent Models of C to U RNA Editing
Valerie Blanc and Nicholas O. Davidson
8. In vivo Analysis of RNA Editing in Plastids
Stephanie Ruf and Ralph Bock
9. Identifying Specific Trans-Factors of RNA Editing in Plant Mitochondria by Multiplex Single Base Extension Typing
Mizuki Takenaka
10. Complementation of Mutants in Plant Mitochondrial RNA Editing by Protoplast Transfection
Mizuki Takenaka and Anja Zehrmann
11. A High Throughput Assay for DNA Deaminases
Meng Wang, Cristina Rada, and Michael S. Neuberger
12. Biochemical Fractionation and Purification of High-Molecular-Mass APOBEC3G Complexes
Ya-Lin Chiu
Part IV: tRNA Editing and RNA Modifications
13. Analysis of tRNA Editing in Native and Synthetic Substrates
Jessica L. Spears, Kirk W. Gaston, and Juan D. Alfonzo
14. Post-Transcriptional Modification of RNAs by Artificial Box H/ACA and Box C/D RNPs
Chao Huang, John Karijolich, and Yi-Tao Yu
15. Functional Analysis of Non-Coding RNAs in Trypanosomes: RNA Walk, A Novel Approach to Study RNA-RNA Interactions Between Small RNA and Its Target
Chaim Wachtel and Shulamit Michaeli
16. A Post-Labeling Approach for the Characterization and Quantification of RNA Modifications Based on Site-Directed Cleavage by DNAzymes
Madeleine Meusburger, Martin Hengesbach, and Mark Helm
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